Anti-MCM2 (phospho S40 + S41) antibody (ab70371)
Key features and details
- Rabbit polyclonal to MCM2 (phospho S40 + S41)
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MCM2 (phospho S40 + S41) antibody
See all MCM2 primary antibodies -
Description
Rabbit polyclonal to MCM2 (phospho S40 + S41) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
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Immunogen
A synthetic di-phosphorylated peptide, corresponding to a sequence from human MCM2 surrounding Serines 40 and 41. (NP_004517.2)
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Positive control
- Whole cell lysate from asynchronous 293T cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MCM2 (phospho S40 + S41) antibody (ab70371)All lanes : Anti-MCM2 (phospho S40 + S41) antibody (ab70371) at 0.1 µg/ml
Lane 1 : Whole cell lysate from asynchronous 293T cells
Lane 2 : Whole cell lysate from asynchronous 293T cells treated with a phosphatase
Lysates/proteins at 50 µg per lane.
Predicted band size: 100 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
The bottom panel shows total MCM2 staining; the blot was stripped and then blotted with rabbit anti-total MCM2 antibody at 0.1 µg/ml. Detection: Chemiluminescence with exposure times of 30 seconds for upper and lower panel. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S40 + S41) antibody (ab70371)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right) tissues labelling MCM2 (phospho S40 + S41) with ab70371 at 1/200 (1µg/ml). Detection: DAB. -
Flow Cytometry - Anti-MCM2 (phospho S40 + S41) antibody (ab70371)ab70371 at 0.1µg/ml staining MCM2 (phospho S40 + S41) by Flow Cytometery. 1x106 cells were fixed in 1.5% PFA and permeabilized in 90% MeOH. DNA content was simultaneously analyzed via PI stain.
