Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3727] to MCM2
Suitable for: Flow Cyt, WB, IP, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-MCM2 antibody [EPR3727]
See all MCM2 primary antibodies
Description

Rabbit monoclonal [EPR3727] to MCM2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa, Jurkat, and MCF-7 cell lysates; Human cervical carcinoma and tonsil tissues; HeLa cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3727
Isotype

IgG
Research areas

Western blot - Anti-MCM2 antibody [EPR3727] (ab133325)

All lanes : Anti-MCM2 antibody [EPR3727] (ab133325) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF-7 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 102 kDa
Flow Cytometry - Anti-MCM2 antibody [EPR3727] (ab133325)

Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MCM2 with unpurified ab133325 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labelling MCM2 with ab133325 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling MCM2 with ab133325 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-MCM2 antibody [EPR3727] (ab133325)

MCM2 was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) with ab133325 at 1/60 dilution.

Exposue time:10 second.
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR3727] (ab133325)

Immunofluorescent analysis of HeLa cells labelling MCM2 with ab133325 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)

Immunohistochemical analysis of paraffin embedded normal Human heart tissue using ab133325 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)

Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab133325 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab133325 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)

Immunohistochemical analysis of paraffin embedded Human Urinary bladder transitional carcinoma tissue using ab133325 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)

Immunohistochemical analysis of paraffin embedded Human Clear cell carcinoma of kidney tissue using ab133325 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (ab133325)

Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab133325 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-MCM2 antibody [EPR3727] (ab133325)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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