Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Wednesday March 10, 2021

Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10819] to MCM2 (phospho S139)
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-MCM2 (phospho S139) antibody [EPR10819]
See all MCM2 primary antibodies
Description

Rabbit monoclonal [EPR10819] to MCM2 (phospho S139)
Host species

Rabbit
Specificity

ab170873 only detects MCM2 phosphorylated at Serine 139.

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MCM2 aa 100-200 (phospho S139) (Cysteine residue). The exact sequence is proprietary.
Database link: P49736
Positive control
- HeLa whole cell lysate (ab150035) treated with Alkaline Phosphatase, Jurkat whole cell lysate (ab7899), Human cervical carcinoma tissue and Human tonsil tissue
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10819
Isotype

IgG
Research areas

Western blot - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)

All lanes : Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873) at 1/1000 dilution

Lane 1 : Hela cell lysate
Lane 2 : Hela cell lysate treated with Alkaline Phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 102 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)

ab170873 showing +ve staining in Human normal colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)

ab170873 showing +ve staining in Human glioma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MCM2 using ab170873 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling MCM2 using ab170873 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)

Western blot analysis on immunoprecipitation pellet from Jurkat lysate using ab170873 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873) at 1/10 dilution + Jurkat cell lysate at 10 µg
Anti-MCM2 (phospho S139) antibody [EPR10819] (ab170873)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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