Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)
![Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)](https://www.abcam.com/ps/products/174/ab174841/Images/ab174841-397644-anti-hpdl-antibody-epr11691-c-terminal-western-blot-human-knockout-lysate.jpg "Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11691] to HPDL - C-terminal
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-HPDL antibody [EPR11691] - C-terminal
See all HPDL primary antibodies -
Description
Rabbit monoclonal [EPR11691] to HPDL - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HPDL aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q96IR7 -
Positive control
- WB: Caco-2, MCF7 and HeLa cell lysates; Human fetal heart lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11691 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)All lanes : Anti-HPDL antibody [EPR11691] - C-terminal (ab174841) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : HPDL knockout HeLa cell lysate
Lane 3 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDaLanes 1-3: Merged signal (red and green). Green - ab174841 observed at 39 kDa. Red - loading control ab7291 observed at 50 kDa.
ab174841 Anti-HPDL antibody [EPR11691] - C-terminal was shown to specifically react with HPDL in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265868 (knockout cell lysate ab257995) was used. Wild-type and HPDL knockout samples were subjected to SDS-PAGE. ab174841 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)](https://www.abcam.com/ps/products/174/ab174841/Images/ab174841-200267-anti-hpdl-antibody-epr11691-c-terminal-western-blot.jpg)
Western blot - Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)All lanes : Anti-HPDL antibody [EPR11691] - C-terminal (ab174841) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa
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![Immunoprecipitation - Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)](https://www.abcam.com/ps/products/174/ab174841/Images/ab174841-200268-anti-hpdl-antibody-epr11691-c-terminal-immunoprecipitation.jpg)
Immunoprecipitation - Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)Western blot analysis on immunoprecipitation pellet from MCF7 cell lysate, labeling HPDL, immunoprecipitated using ab174841 at 1/10 dilution.
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![Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)](https://www.abcam.com/ps/products/174/ab174841/Images/ab174841-4-benefits-of-recombinant-antibodies.png)
Anti-HPDL antibody [EPR11691] - C-terminal (ab174841)
