Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4120] to MCM2
Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-MCM2 antibody [EPR4120]
See all MCM2 primary antibodies
Description

Rabbit monoclonal [EPR4120] to MCM2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Rat Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MCF-7, Ramos, SW480, Molt-4, Jurkat and HeLa whole cell lysate (ab150035) IHC-P: Human squamous cell cervical carcinoma tissue and Human tonsil tissue IF: HeLa cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 5.80 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4120
Isotype

IgG
Research areas

Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)

All lanes : Anti-MCM2 antibody [EPR4120] (ab108935) at 1/10000 dilution (unpurified)

Lane 1 : MCF-7 cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : Molt-4 cell lysate
Lane 5 : Jurkat cell lysate
Lane 6 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 102 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)

Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labeling MCM2 with purified ab108935 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)

Immunohistochemical staining of MCM2 in paraffin-embedded human tonsil tissue with unpurified ab108395, at a 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)

Unpurified ab108935, at a 1/100 dilution, staining MCM2 in HeLa cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)

Immunohistochemical staining of paraffin embedded human tonsil with purified ab108935 at a working dilution of 1 in 200. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)

Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with purified ab108935 at a dilution of 1/600. An Alexa Fluor^® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)

All lanes : Anti-MCM2 antibody [EPR4120] (ab108935) at 1/8500 dilution (purified)

Lane 1 : HeLa cell lysate
Lane 2 : Mouse kidney tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 102 kDa
Observed band size: 125 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)

Immunohistochemical staining of paraffin embedded rat liver with purified ab108935 at a working dilution of 1 in 200. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)

Anti-MCM2 antibody [EPR4120] (ab108935) at 1/8500 dilution (purified) + Jurkat cell lysate at 10 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 102 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Flow Cytometry - Anti-MCM2 antibody [EPR4120] (ab108935)

Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MCM2 with unpurified ab108935 at 1/160 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
Western blot - Anti-MCM2 antibody [EPR4120] (ab108935)

All lanes : Anti-MCM2 antibody [EPR4120] (ab108935) at 1/5000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : Mouse kidney tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L)

Predicted band size: 102 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)

Immunohistochemical staining of MCM2 in paraffin-embedded human squamous cell cervical carcinoma tissue with unpurified ab108395, at a 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR4120] (ab108935)

Immunohistochemical staining of paraffin embedded rat liver with unpurified ab108935 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (ab108935)

Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with unpurified ab108935 at a dilution of 1/250. An Alexa Fluor^® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
OI-RD Scanning - Anti-MCM2 antibody [EPR4120] (ab108935)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-MCM2 antibody [EPR4120] (ab108935)

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