Anti-MCM2 antibody - N-terminal (ab227278)
Key features and details
- Rabbit polyclonal to MCM2 - N-terminal
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MCM2 antibody - N-terminal
See all MCM2 primary antibodies -
Description
Rabbit polyclonal to MCM2 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment within Human MCM2 (N terminal). The exact sequence is proprietary.
Database link: P49736 -
Positive control
- WB: HCT 116 whole cell lysate. IP: HCT 116 whole cell extract. ICC/IF: HCT 116 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MCM2 antibody - N-terminal (ab227278)Anti-MCM2 antibody - N-terminal (ab227278) at 1/1000 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 102 kDa7.5% SDS-PAGE gel.
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Immunoprecipitation - Anti-MCM2 antibody - N-terminal (ab227278)MCM2 was immunoprecipitated from HCT 116 (human colorectal carcinoma cell line) whole cell lysate with 4 μg ab227278. Western blot was performed from the immunoprecipitate using ab227278 at 1/500 dilution.
Lane 1: HCT 116 whole cell extract 40 μg (Input).
Lane 2: Preimmune Rabbit IgG instead of ab227278 in HCT 116 whole cell extract.
Lane 3: ab227278 IP in HCT 116 whole cell extract.
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Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody - N-terminal (ab227278)4% paraformaldehyde-fixed HCT 116 (human colorectal carcinoma cell line) cells stained for MCM2 (green) using ab227278 at 1/500 dilution in ICC/IF. Counterstain: Phalloidin cytoskeletal marker at 1/50 dilution.
