Recombinant rhesus monkey CD4 protein (Fc Chimera Active) (ab201425)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE
Preparation and Storage
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Alternative names
- CD 4
- CD4
- CD4 (L3T4)
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Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
Images
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SDS-PAGE - Recombinant Rhesus monkey CD4 protein (Fc Chimera) (ab201425)
The purity of ab201425 was determined by DTT-reduced (R) SDS-PAGE and staining overnight with Coomassie Blue. DTT-reduced Protein migrates as 67-85 kDa in SDS-PAGE due to glycosylation.
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ELISA - Recombinant rhesus monkey CD4 protein (Fc Chimera Active) (ab201425)Immobilized Recombinant Human HIV-1 gp120 (CN54) protein (ab174070) at 10 μg/mL (100 μL/well) can bind ab201425 with a linear range of 0.04-0.31 μg/mL.
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ELISA - Recombinant rhesus monkey CD4 protein (Fc Chimera Active) (ab201425)Immobilized Recombinant Rhesus monkey CD4 (Fc Chimera) (ab201425) at 1 μg/mL (100 μL/well) can bind Ibalizumab with a linear range of 0.4-25 ng/mL.
