Antibodies, Proteins, Kits and Reagents for Life Science

Recombinant rhesus monkey CD4 protein (Fc Chimera Active) (ab201425)

Key features and details

Expression system: HEK 293 cells
Purity: > 95% SDS-PAGE
Endotoxin level:
Active: Yes
Suitable for: SDS-PAGE

Alternative names
- CD 4
- CD4
- CD4 (L3T4)
- CD4 antigen
- CD4 antigen (p55)
- CD4 molecule
- CD4 receptor
- CD4+ Lymphocyte deficiency, included
- CD4_HUMAN
- CD4mut
- L3T4
- Leu3
- Ly-4
- Lymphocyte antigen CD4
- MGC165891
- OTTHUMP00000238897
- p55
- T cell antigen T4
- T cell antigen T4/LEU3
- T cell differentiation antigen L3T4
- T cell OKT4 deficiency, included
- T cell surface antigen T4/Leu 3
- T cell surface antigen T4/Leu3
- T cell surface glycoprotein CD4
- T-cell surface antigen T4/Leu-3
- T-cell surface glycoprotein CD4
- W3/25
- W3/25 antigen
see all
Function

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Sequence similarities

Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Post-translational
modifications

Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localization

Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Target information above from: UniProt accession P01730 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

SDS-PAGE - Recombinant Rhesus monkey CD4 protein (Fc Chimera) (ab201425)

The purity of ab201425 was determined by DTT-reduced (R) SDS-PAGE and staining overnight with Coomassie Blue. DTT-reduced Protein migrates as 67-85 kDa in SDS-PAGE due to glycosylation.
ELISA - Recombinant rhesus monkey CD4 protein (Fc Chimera Active) (ab201425)

Immobilized Recombinant Human HIV-1 gp120 (CN54) protein (ab174070) at 10 μg/mL (100 μL/well) can bind ab201425 with a linear range of 0.04-0.31 μg/mL.
ELISA - Recombinant rhesus monkey CD4 protein (Fc Chimera Active) (ab201425)

Immobilized Recombinant Rhesus monkey CD4 (Fc Chimera) (ab201425) at 1 μg/mL (100 μL/well) can bind Ibalizumab with a linear range of 0.4-25 ng/mL.

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