Anti-MCM2 (phospho S53) antibody (ab70367)
Key features and details
- Rabbit polyclonal to MCM2 (phospho S53)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MCM2 (phospho S53) antibody
See all MCM2 primary antibodies -
Description
Rabbit polyclonal to MCM2 (phospho S53) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseHumanWB Human -
Immunogen
A synthetic phospho-peptide, corresponding to a sequence from human MCM2 surrounding Serine 53 (NP_004517.2)
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Positive control
- Whole cell lysate from asynchronous 293T cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling MCM2 (phospho S53) with ab70367 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-MCM2 (phospho S53) antibody (ab70367) at 0.1 µg/ml
Lane 1 : Whole cell lysate from asynchronous 293T cells
Lane 2 : Whole cell lysate from asynchronous 293T cells treated with a phosphatase.
Lysates/proteins at 50 µg per lane.
Predicted band size: 100 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
The bottom panel shows total MCM2 staining; the blot was stripped and then blotted with rabbit anti-total MCM2 antibody at 0.1 µg/ml. Detection: Chemiluminescence with exposure times of 3 minutes (upper panel) and 10 seconds (lower panel).