Anti-PBK/SPK antibody (ab227239)
Key features and details
- Rabbit polyclonal to PBK/SPK
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PBK/SPK antibody
See all PBK/SPK primary antibodies -
Description
Rabbit polyclonal to PBK/SPK -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment within Human PBK/SPK (internal sequence). The exact sequence is proprietary.
Database link: Q96KB5 -
Positive control
- WB: A431 and HeLa whole cell extracts. IP: A431 whole cell extract.
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General notes
This product was previously labelled as PBK
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PBK/SPK antibody (ab227239)All lanes : Anti-PBK/SPK antibody (ab227239) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa10% SDS-PAGE gel.
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Immunoprecipitation - Anti-PBK/SPK antibody (ab227239)PBK/SPK was immunoprecipitated from A431 (human epidermoid carcinoma cell line) whole cell extract with 5 µg ab227239. Western blot was performed from the immunoprecipitate using ab227239. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: A431 whole cell extract.
Lane 1: Control IgG IP in A431 whole cell extract.
Lane 2: ab227239 IP in A431 whole cell extract.
