Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Wednesday March 10, 2021

Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2475(N)] to PBK/SPK (phospho T9)
Suitable for: IHC-P, Flow Cyt, WB
Reacts with: Rat, Human

Product name

Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)]
See all PBK/SPK primary antibodies
Description

Rabbit monoclonal [EPR2475(N)] to PBK/SPK (phospho T9)
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Rat Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa cells/lysate treated with Nocodazole. IHC-P: Human testis and rat spleen tissues. Flow Cyt: HeLa cells.
General notes
This product was previously labelled as PBK

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2475(N)
Isotype

IgG
Research areas

Western blot - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)

All lanes : Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953) at 1/5000 dilution

Lane 1 : HeLa call lysate treated with Nocodazole
Lane 2 : HeLa cell lysate untreated

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?
Flow Cytometry - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilised HeLa (Human cervix adenocarcinoma epithelial cell) treated with 0.1 µg/mL Nocodazole for 24h (Red) / Untreated control (Green) cells labeling PBK/SPK (phospho T9) with ab184953 at 1/800 dilution compared with a Isotype control Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Higher expression in part of Nocodazole treated samples (mitotic cell induction) which is consistent with cell cycle Flow Cytometry data.
Flow Cytometry - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)

Flow cytometric analysis of 4% paraformaldehyde fixed HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PBK/SPK (phospho T9) with ab184953 at 1/800 dilution (Right) compared with a Rabbit monoclonal IgG Isotype control (ab172730) (Left). Cells permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Highest expression at M phase.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling PBK/SPK (phospho T9) with ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling PBK/SPK (phospho T9) with ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)

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