Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)
![Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)](https://www.abcam.com/ps/products/184/ab184953/Images/ab184953-217645-anti-pbk-phospho-t9-antibody-epr2475n-western-blot.jpg "Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2475(N)] to PBK/SPK (phospho T9)
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)]
See all PBK/SPK primary antibodies -
Description
Rabbit monoclonal [EPR2475(N)] to PBK/SPK (phospho T9) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P RatHumanWB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa cells/lysate treated with Nocodazole. IHC-P: Human testis and rat spleen tissues. Flow Cyt: HeLa cells.
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General notes
This product was previously labelled as PBK
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2475(N) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)All lanes : Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953) at 1/5000 dilution
Lane 1 : HeLa call lysate treated with Nocodazole
Lane 2 : HeLa cell lysate untreated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
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![Flow Cytometry - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)](https://www.abcam.com/ps/products/184/ab184953/Images/ab184953-396516-anti-pbk-spk-phospho-t9-antibody-epr2475n-flow-cytometry.jpg)
Flow Cytometry - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilised HeLa (Human cervix adenocarcinoma epithelial cell) treated with 0.1 µg/mL Nocodazole for 24h (Red) / Untreated control (Green) cells labeling PBK/SPK (phospho T9) with ab184953 at 1/800 dilution compared with a Isotype control Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Higher expression in part of Nocodazole treated samples (mitotic cell induction) which is consistent with cell cycle Flow Cytometry data.
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![Flow Cytometry - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)](https://www.abcam.com/ps/products/184/ab184953/Images/ab184953-396515-anti-pbk-spk-phospho-t9-antibody-epr2475-n-flow-cytometry.jpg)
Flow Cytometry - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)Flow cytometric analysis of 4% paraformaldehyde fixed HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PBK/SPK (phospho T9) with ab184953 at 1/800 dilution (Right) compared with a Rabbit monoclonal IgG Isotype control (ab172730) (Left). Cells permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Highest expression at M phase.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)](https://www.abcam.com/ps/products/184/ab184953/Images/ab184953-217647-anti-pbk-phospho-t9-antibody-epr2475n-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)Immunohistochemical analysis of paraffin-embedded human testis tissue labeling PBK/SPK (phospho T9) with ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)](https://www.abcam.com/ps/products/184/ab184953/Images/ab184953-217648-anti-pbk-phospho-t9-antibody-epr2475n-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling PBK/SPK (phospho T9) with ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)](https://www.abcam.com/ps/products/184/ab184953/Images/ab184953-7-benefits-of-recombinant-antibodies.png)
Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (ab184953)
