Granzyme B Inhibitor Screening Assay Kit (ab157405)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
Overview
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Product name
Granzyme B Inhibitor Screening Assay Kit
See all Granzyme B kits -
Detection method
Fluorescent -
Assay type
Quantitative -
Product overview
In Abcam's Granzyme B Inhibitor Screening Assay Kit, active human Granzyme B enzyme hydrolyzes the specific substrate to release the quench of fluorescent group, which can be detected fluorometrically at Ex/Em = 380/500 nm. In the presence of potent Granzyme B Inhibitor, the hydrolyzation of substrate will be impeded. The Kit provides a rapid, simple, sensitive and reliable test suitable for high throughput screening of Granzyme B inhibitors or characterize/study Granzyme B inhibitors. Inhibitor control, Ac-IEPD-CHO is included to compare the efficacy of test inhibitors.
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Notes
Granzyme B (GZMB, EC number 3.4.21.79) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Granzyme B Assay Buffer WM 1 x 25ml Granzyme B Enzyme Green 1 vial Granzyme B Substrate Red 1 x 0.5ml Inhibitor Control (Ac-IEPD-CHO) Blue 1 x 20µl -
Research areas
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Function
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. -
Sequence similarities
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells. - Information by UniProt
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Alternative names
- C11
- Cathepsin G like 1
- Cathepsin G-like 1
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Images
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Inhibition of Granzyme B ActivityInhibition of Granzyme B Activity by Granzyme B Inhibitor, Ac-IEPD-CHO. Assay was performed following Kit protocol.
