Anti-Ran antibody - C-terminal (ab226205)
Key features and details
- Rabbit polyclonal to Ran - C-terminal
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Ran antibody - C-terminal
See all Ran primary antibodies -
Description
Rabbit polyclonal to Ran - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog, Xenopus laevis, Orangutan
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Immunogen
Synthetic peptide within Human Ran aa 175-216 (C terminal). The exact sequence is proprietary. NP_006316.1
Database link: P62826 -
Positive control
- IHC-P: Human breast carcinoma and mouse renal cell carcinoma tissue. IP: HEK-293T whole cell lysate. WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ran antibody - C-terminal (ab226205)All lanes : Anti-Ran antibody - C-terminal (ab226205) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 24 kDa
Exposure time: 3 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody - C-terminal (ab226205)Formalin fixed, paraffin embedded human breast carcinoma tissue stained for Ran with ab226205 (1/5000 dilution) in immunohistochemical analysis. DAB detection.
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Immunoprecipitation - Anti-Ran antibody - C-terminal (ab226205)Ran was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226205 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226205 at 1 µg/ml.
Lane 1: ab226205 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ran antibody - C-terminal (ab226205)Formalin fixed, paraffin embedded mouse renal cell carcinoma tissue stained for Ran with ab226205 (1/5000 dilution) in immunohistochemical analysis. DAB detection.
