Anti-Mitochondrial Ferritin antibody (ab66111)
Key features and details
- Rabbit polyclonal to Mitochondrial Ferritin
- Suitable for: WB, IHC-Fr, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Mitochondrial Ferritin antibody
See all Mitochondrial Ferritin primary antibodies -
Description
Rabbit polyclonal to Mitochondrial Ferritin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Chimpanzee, Macaque monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human Mitochondrial Ferritin aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q8N4E7 -
Positive control
- This antibody gave a positive signal in both human liver and brain tissue lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab66111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 27 kDa). IHC-Fr 1/100. ICC/IF 1/100. Target
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Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. -
Tissue specificity
Detected in testis and erythroleukemia. Expression is very low or not detectable in brain, colon, heart, kidney, liver, lung, muscle, placental, spleen and small intestine. -
Sequence similarities
Belongs to the ferritin family.
Contains 1 ferritin-like diiron domain. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 94033 Human
- Entrez Gene: 67634 Mouse
- Omim: 608847 Human
- SwissProt: Q2YDI9 Cow
- SwissProt: Q8N4E7 Human
- SwissProt: Q9D5H4 Mouse
- Unigene: 105324 Human
- Unigene: 179144 Mouse
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Alternative names
- FerH antibody
- Ferritin antibody
- Ferritin H subunit antibody
see all
Images
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Western blot - Anti-Mitochondrial Ferritin antibody (ab66111)All lanes : Anti-Mitochondrial Ferritin antibody (ab66111) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
The band observed at 21 kDa could potentially be a cleaved form of Human Ferritin, mitochondrial precursor due to a 49 amino acid transit peptide (SwissProt), which when cleaved gives an expected Mw of 22 kDa. -
Immunohistochemistry (Frozen sections) - Anti-Mitochondrial Ferritin antibody (ab66111)This image is courtesy of Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.IHC-Fr image of Ferritin, mitochondrial staining on mouse skeletal muscle using ab66111 (1: 100). The sections were fixed in 4% PFA and the section was permeabilized using 0.1% TritonX in 0.1% PBS. The section was then blocked using 10% donkey serum for 1 hour at 24°C. The primary antibody (1:100) was incubated at 4°C for 24 hours and the secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).
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Immunocytochemistry/ Immunofluorescence - Anti-Mitochondrial Ferritin antibody (ab66111)This image is courtesy of Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.ICC/IF image of Ferritin, mitochondrial staining on mouse hippocampal culture using ab66111 (1: 100). The cells were fixed in 4% PFA and the section was permeabilized using 0.1% TritonX in 0.1% PBS. The cells was then blocked using 10% donkey serum for 1 hour at 24°C. The primary antibody (1:100) was incubated at 4°C for 24 hours and the secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).
Protocols
Datasheets and documents
References (1)
ab66111 has been referenced in 1 publication.
- Gong S et al. Roflumilast restores cAMP/PKA/CREB signaling axis for FtMt-mediated tumor inhibition of ovarian cancer. Oncotarget 8:112341-112353 (2017). PubMed: 29348829
Images
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Western blot - Anti-Mitochondrial Ferritin antibody (ab66111)All lanes : Anti-Mitochondrial Ferritin antibody (ab66111) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
The band observed at 21 kDa could potentially be a cleaved form of Human Ferritin, mitochondrial precursor due to a 49 amino acid transit peptide (SwissProt), which when cleaved gives an expected Mw of 22 kDa. -
Immunohistochemistry (Frozen sections) - Anti-Mitochondrial Ferritin antibody (ab66111) This image is courtesy of Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.
IHC-Fr image of Ferritin, mitochondrial staining on mouse skeletal muscle using ab66111 (1: 100). The sections were fixed in 4% PFA and the section was permeabilized using 0.1% TritonX in 0.1% PBS. The section was then blocked using 10% donkey serum for 1 hour at 24°C. The primary antibody (1:100) was incubated at 4°C for 24 hours and the secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).
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Immunocytochemistry/ Immunofluorescence - Anti-Mitochondrial Ferritin antibody (ab66111) This image is courtesy of Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.
ICC/IF image of Ferritin, mitochondrial staining on mouse hippocampal culture using ab66111 (1: 100). The cells were fixed in 4% PFA and the section was permeabilized using 0.1% TritonX in 0.1% PBS. The cells was then blocked using 10% donkey serum for 1 hour at 24°C. The primary antibody (1:100) was incubated at 4°C for 24 hours and the secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).
