Anti-Mitochondrial Ferritin antibody (ab93428)
Key features and details
- Rabbit polyclonal to Mitochondrial Ferritin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Mitochondrial Ferritin antibody
See all Mitochondrial Ferritin primary antibodies -
Description
Rabbit polyclonal to Mitochondrial Ferritin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
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Positive control
- This antibody gave a positive signal in both Hela and HEK293 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB 1/250. Detects a band of approximately 22 kDa (predicted molecular weight: 27 kDa).Notes WB
1/250. Detects a band of approximately 22 kDa (predicted molecular weight: 27 kDa).Target
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Function
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. -
Tissue specificity
Detected in testis and erythroleukemia. Expression is very low or not detectable in brain, colon, heart, kidney, liver, lung, muscle, placental, spleen and small intestine. -
Sequence similarities
Belongs to the ferritin family.
Contains 1 ferritin-like diiron domain. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 94033 Human
- Omim: 608847 Human
- SwissProt: Q8N4E7 Human
- Unigene: 105324 Human
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Alternative names
- FerH antibody
- Ferritin antibody
- Ferritin H subunit antibody
see all
Images
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Western blot - Anti-Mitochondrial Ferritin antibody (ab93428)All lanes : Anti-Mitochondrial Ferritin antibody (ab93428) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutes
The band observed at 22 kDa could potentially be a cleaved form of Human Ferritin, mitochondrial precursor due to a 49 amino acid transit peptide (SwissProt), which when cleaved gives an expected Mw of 22 kDa.
Datasheets and documents
References (0)
ab93428 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Mitochondrial Ferritin antibody (ab93428)All lanes : Anti-Mitochondrial Ferritin antibody (ab93428) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutes
The band observed at 22 kDa could potentially be a cleaved form of Human Ferritin, mitochondrial precursor due to a 49 amino acid transit peptide (SwissProt), which when cleaved gives an expected Mw of 22 kDa.
