Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Factor H antibody [EPR6225] (ab133536)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6225] to Factor H
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Factor H antibody [EPR6225]
    See all Factor H primary antibodies

  • Description

    Rabbit monoclonal [EPR6225] to Factor H

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Factor H aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: A549 and HaCaT cell lysates; Human plasma, kidney, fetal lung, and fetal liver lysates; Purified Factor H protein.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 9.40 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR6225

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Factor H antibody [EPR6225] (ab133536)
    Western blot - Anti-Factor H antibody [EPR6225] (ab133536)
    All lanes : Anti-Factor H antibody [EPR6225] (ab133536) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : CFH knockout A549 cell lysate
    Lane 3 : HaCaT cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 139 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab133536 observed at 180 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab133536 Anti-Factor H antibody [EPR6225] was shown to specifically react with Factor H in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267031 (knockout cell lysate ab257150) was used. Wild-type and Factor H knockout samples were subjected to SDS-PAGE. ab133536 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Factor H antibody [EPR6225] (ab133536)
    Western blot - Anti-Factor H antibody [EPR6225] (ab133536)
    All lanes : Anti-Factor H antibody [EPR6225] (ab133536) at 1/1000 dilution

    Lane 1 : Purified Factor H protein at 0.5 µg
    Lane 2 : Purified Factor H protein at 0.1 µg

    Performed under reducing conditions.

    Predicted band size: 139 kDa
    Observed band size: 170 kDa why is the actual band size different from the predicted?



    This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab133536 overnight at 4°C at a 1/1000 dilution. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Factor H antibody [EPR6225] (ab133536)
    Western blot - Anti-Factor H antibody [EPR6225] (ab133536)
    All lanes : Anti-Factor H antibody [EPR6225] (ab133536) at 1/1000 dilution

    Lane 1 : Human plasma lysate
    Lane 2 : Human kidney lysate
    Lane 3 : Human fetal lung lysate
    Lane 4 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 139 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-Factor H antibody [EPR6225] (ab133536)
    OI-RD Scanning - Anti-Factor H antibody [EPR6225] (ab133536)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Factor H antibody [EPR6225] (ab133536)
    Anti-Factor H antibody [EPR6225] (ab133536)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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