Anti-Fibronectin antibody (ab299)
Key features and details
- Rabbit polyclonal to Fibronectin
- Suitable for: ICC/IF, Sandwich ELISA
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Fibronectin antibody
See all Fibronectin primary antibodies -
Description
Rabbit polyclonal to Fibronectin -
Host species
Rabbit -
Specificity
This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Minimum Cross Reactivity to collagens and non-collagen extracellular matrix proteins. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumansELISA Human
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Immunogen
Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. This product has been sterile filtered. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Sandwich ELISA - Anti-Fibronectin antibody (ab299)Standard Curve for Fibronectin (Analyte: Fibronectin protein (Human) (ab81743)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [HFN 7.1] to Fibronectin - BSA and Azide free (ab80923) at 1µg/ml and Detector Antibody Rabbit polyclonal to Fibronectin (ab299) at 0.5µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody (ab299)ICC/IF image of ab299 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab299, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
