Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22699-151] to Laminin gamma 3
  • Suitable for: IHC-Fr, IHC-P, WB, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Laminin gamma 3 antibody [EPR22699-151]
    See all Laminin gamma 3 primary antibodies

  • Description

    Rabbit monoclonal [EPR22699-151] to Laminin gamma 3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse testis and Mouse kidney lysates. IHC-P: Mouse testis, Mouse cerebrum and Mouse lung tissues. Flow Cyt: RAW 264.7 cells. IHC-fr: Mouse testis tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429)

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Laminin gamma 3 with ab234429 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the basement membrane in the mouse lung is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429)
    Western blot - Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429)
    All lanes : Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429) at 1/10000 dilution

    Lane 1 : Mouse testis tissue lysate 20 µg
    Lane 2 : Mouse kidney tissue lysate 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 171 kDa
    Observed band size: 172 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST   Exposure time: 3 minutes 

  • Immunohistochemistry (Frozen sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429)
    Immunohistochemistry (Frozen sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse testis tissue labeling Laminin gamma 3 with Ab234429 at 1/100 (4.99 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

  • Flow Cytometry - Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429)
    Flow Cytometry - Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Laminin gamma 3 with ab234429 at 1/50 (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Laminin gamma 3 with ab234429 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the blood vessels in the mouse cerebrum (PMID:15824114) is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Laminin gamma 3 with ab234429 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the basement membrane in the mouse testis (PMID: 15824114) is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429)
    Anti-Laminin gamma 3 antibody [EPR22699-151] (ab234429)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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