Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Robo4 antibody [EPR23426-145] - BSA and Azide free (ab273519)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23426-145] to Robo4 - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Robo4 antibody [EPR23426-145] - BSA and Azide free
    See all Robo4 primary antibodies

  • Description

    Rabbit monoclonal [EPR23426-145] to Robo4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HUVEC whole cell lysate. Flow Cyt: HUVEC cells.

  • General notes

    ab273519 is the carrier-free version of ab272734. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-Robo4 antibody [EPR23426-145] - BSA and Azide free (ab273519)
    Flow Cytometry - Anti-Robo4 antibody [EPR23426-145] - BSA and Azide free (ab273519)

    Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell) (Left panel) / HUVEC (Human umbilical vein endothelial cell) (Right panel) cells labelling Robo4 with ab272734 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

    Negative control: HeLa (PMID: 11076864).

    Gated on viable cells.

    Brighter secondary antibody like Alexa Fluor® 647 is required to get desirable staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272734).

  • Anti-Robo4 antibody [EPR23426-145] - BSA and Azide free (ab273519)
    Anti-Robo4 antibody [EPR23426-145] - BSA and Azide free (ab273519)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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