Anti-Factor H antibody [OX-24] - BSA and Azide free (ab244574)
![Anti-Factor H antibody [OX-24] - BSA and Azide free (ab244574)](https://www.abcam.com/ps/products/244/ab244574/Images/ab244574-356450-AntiFactor-H-antibody-OX24-western-blot-serum-plasma-purified-protein-human.jpg "Anti-Factor H antibody [OX-24] - BSA and Azide free (ab244574)")
Key features and details
- Mouse monoclonal [OX-24] to Factor H - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Factor H antibody [OX-24] - BSA and Azide free
See all Factor H primary antibodies -
Description
Mouse monoclonal [OX-24] to Factor H - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- WB: Human plasma, Human serum, Purified Factor H protein
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General notes
ab244574 is the PBS only version of ab118820.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
OX-24 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Factor H antibody [OX-24] - BSA and Azide free (ab244574)All lanes : Anti-Factor H antibody [OX-24] (ab118820) at 1 µg/ml
Lane 1 : Human serum diluted 1/1000 at 10 µl
Lane 2 : Human plasma diluted 1/1000 at 10 µl
Lane 3 : Purified Factor H protein at 0.5 µg
Lane 4 : Purified Factor H protein at 0.1 µg
Performed under reducing conditions.
Predicted band size: 139 kDaThis image was produced using the same antibody clone but in a different formulation (ab118820).
This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab118820 overnight at 4°C at a 1µg/ml concentration. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/20000 dilution for 1 hour at room temperature before imaging.
