Anti-Hsp27 antibody [5D7] (ab114067)
![Anti-Hsp27 antibody [5D7] (ab114067)](https://www.abcam.com/ps/datasheet/images/114/ab114067/Hsp27-Primary-antibodies-ab114067-1.jpg "Anti-Hsp27 antibody [5D7] (ab114067)")
Key features and details
- Mouse monoclonal [5D7] to Hsp27
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Hsp27 antibody [5D7]
See all Hsp27 primary antibodies -
Description
Mouse monoclonal [5D7] to Hsp27 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
Purified recombinant fragment of human Hsp27 expressed in E. Coli.
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Positive control
- WB: Hela; A549; Jurkat; A431; HEK293; HepG2 and PC-12 cell lysates. IHC-P: Brain tissues; esophageal cancer tissues.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR146269-8 are from Tissue Culture Supernatant
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
5D7 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Hsp27 antibody [5D7] (ab114067)All lanes : Anti-Hsp27 antibody [5D7] (ab114067) at 1/500 dilution
Lane 1 : Hela cell lysate
Lane 2 : A549 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : HepG2 cell lysate
Lane 7 : PC-12 cell lysate
Predicted band size: 27 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody [5D7] (ab114067)](https://www.abcam.com/ps/datasheet/images/114/ab114067/Hsp27-Primary-antibodies-ab114067-4.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody [5D7] (ab114067)Immunofluorescence analysis of Hela cells using ab114067 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [5D7] (ab114067)](https://www.abcam.com/ps/datasheet/images/114/ab114067/Hsp27-Primary-antibodies-ab114067-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [5D7] (ab114067)Immunohistochemical analysis of paraffin embedded breast cancer tissues (left) and cardiac muscle tissues (right) using ab114067 at 1/200 dilution with DAB staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [5D7] (ab114067)](https://www.abcam.com/ps/datasheet/images/114/ab114067/Hsp27-Primary-antibodies-ab114067-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp27 antibody [5D7] (ab114067)Immunohistochemical analysis of paraffin embedded brain tissues (left) and esophageal cancer tissues (right) using ab114067 at 1/200 dilution with DAB staining. -
![Flow Cytometry - Anti-Hsp27 antibody [5D7] (ab114067)](https://www.abcam.com/ps/datasheet/images/114/ab114067/Hsp27-Primary-antibodies-ab114067-5.jpg)
Flow Cytometry - Anti-Hsp27 antibody [5D7] (ab114067)Flow cytometric analysis of HepG2 cells using ab114067 at 1/200 dilution (green) and negative control (purple).
