Anti-Hsc70 antibody (ab154415)
Key features and details
- Rabbit polyclonal to Hsc70
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Hsc70 antibody
See all Hsc70 primary antibodies -
Description
Rabbit polyclonal to Hsc70 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Zebrafish -
Immunogen
Recombinant protein fragment, corresponding to a region within amino acids 470-635 of Human Hsc70 (Uniprot ID: P11142).
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Positive control
- Recombinant human Hsc70 protein (ab78431) can be used as a positive control in WB. 293T, A431, H1299, HepG2, Molt-4 and Raji cell lysates; Mouse brain whole cell lysate; HeLa cells; Human U87 xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-Hsc70 antibody (ab154415) at 1/5000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 70 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Hsc70 using ab154415 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human U87 xenograft tissue, labeling Hsc70 using ab154415 at 1/100 dilution.
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Anti-Hsc70 antibody (ab154415) at 1/10000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 70 kDa
7.5% SDS PAGE