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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-Hsc70 antibody (ab154415)

Anti-Hsc70 antibody (ab154415)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Hsc70
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Hsc70 antibody
    See all Hsc70 primary antibodies
  • Description

    Rabbit polyclonal to Hsc70
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Zebrafish
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 470-635 of Human Hsc70 (Uniprot ID: P11142).

  • Positive control

    • Recombinant human Hsc70 protein (ab78431) can be used as a positive control in WB. 293T, A431, H1299, HepG2, Molt-4 and Raji cell lysates; Mouse brain whole cell lysate; HeLa cells; Human U87 xenograft tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins

Images

  • Western blot - Anti-Hsc70 antibody (ab154415)
    Western blot - Anti-Hsc70 antibody (ab154415)
    Anti-Hsc70 antibody (ab154415) at 1/5000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 70 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody (ab154415)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsc70 antibody (ab154415)
    Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Hsc70 using ab154415 at 1/200 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody (ab154415)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody (ab154415)
    Immunohistochemical analysis of paraffin-embedded Human U87 xenograft tissue, labeling Hsc70 using ab154415 at 1/100 dilution.
  • Western blot - Anti-Hsc70 antibody (ab154415)
    Western blot - Anti-Hsc70 antibody (ab154415)
    Anti-Hsc70 antibody (ab154415) at 1/10000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 70 kDa



    7.5% SDS PAGE

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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