Anti-Hsp27 antibody (ab231552)
Key features and details
- Rabbit polyclonal to Hsp27
- Suitable for: WB, ICC/IF
- Reacts with: Cow, Human
- Isotype: IgG
Overview
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Product name
Anti-Hsp27 antibody
See all Hsp27 primary antibodies -
Description
Rabbit polyclonal to Hsp27 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Cow
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Immunogen
Recombinant full length protein (His-tag) corresponding to Cow Hsp27 aa 1 to the C-terminus. (Expressed in E.coli).
Database link: Q3T149 -
Positive control
- ICC/IF: HeLa cells. WB: Cow serum; Cow heart and skeletal muscle lysates; HeLa cell lysate; Recombinant cow Hsp27 protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab231552 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Hsp27 antibody (ab231552)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hsp27 (green) using ab231552 at 20 µg/ml in ICC/IF, followed by an FITC conjugated secondary antibody.
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Western blot - Anti-Hsp27 antibody (ab231552)Anti-Hsp27 antibody (ab231552) at 3 µg/ml + Recombinant cow Hsp27 protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 22 kDa
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Western blot - Anti-Hsp27 antibody (ab231552)All lanes : Anti-Hsp27 antibody (ab231552) at 3 µg/ml
Lane 1 : Cow serum
Lane 2 : Cow heart lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 4 : Cow skeletal muscle lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 22 kDa
