Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)
![Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)](https://www.abcam.com/ps/products/269/ab269858/Images/ab269858-412169-anti-gsdmc1-antibody-epr2088750-western-blot-lysate.jpg "Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20887-50] to GSDMC - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse
Overview
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Product name
Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free
See all GSDMC primary antibodies -
Description
Rabbit monoclonal [EPR20887-50] to GSDMC - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse stomach and esophagus tissue lysates; HEK-293T transfected with mGSDMC1 expression vector, whole cell lysate.
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General notes
The gene for mouse GSDMC is also known as GSDMC1
ab269858 is the carrier-free version of ab225635. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab269858 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20887-50 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)All lanes : Anti-GSDMC antibody [EPR20887-50] (ab225635) at 1/1000 dilution
Lane 1 : Mouse stomach tissue lysate
Lane 2 : Mouse esophagus tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?This data was developed using ab225635, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure times: Lane 1: 15 secondsLane 2: 3.25 seconds.
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![Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)](https://www.abcam.com/ps/products/269/ab269858/Images/ab269858-412170-anti-gsdmc1-antibody-epr2088750-western-blot-tissue.jpg)
Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)All lanes : Anti-GSDMC antibody [EPR20887-50] (ab225635) at 1/1000 dilution
Lane 1 : Wild-type Mouse esophagus tissue lysate
Lane 2 : mGSDMC1-4 knockout mouse esophagus tissue lysate
Lane 3 : Wild-type Mouse stomach tissue lysate
Lane 4 : mGSDMC1-4 knockout mouse stomach tissue lysate
Lane 5 : Wild-type Mouse small intestine tissue lysate
Lane 6 : mGSDMC1-4 knockout mouse small intestine tissue lysate
Lane 7 : Wild-type Mouse colon tissue lysate
Lane 8 : mGSDMC1-4 knockout mouse colon tissue lysate
Lane 9 : Wild-type Mouse caecum tissue lysate
Lane 10 : mGSDMC1-4 knockout mouse caecum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?This data was developed using ab225635, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).
Exposure time: 60 seconds.
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![Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)](https://www.abcam.com/ps/products/269/ab269858/Images/ab269858-412171-anti-gsdmc1-antibody-epr2088750-western-blot-kidney.jpg)
Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)All lanes : Anti-GSDMC antibody [EPR20887-50] (ab225635) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), whole cell lysate
Lane 2 : HEK-293T transfected with mGSDMC1 expression vector, whole cell lysate
Lane 3 : HEK-293T transfected with mGSDMC2 expression vector, whole cell lysate
Lane 4 : HEK-293T transfected with mGSDMC3 expression vector, whole cell lysate
Lane 5 : HEK-293T transfected with mGSDMC4 expression vector, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?This data was developed using ab225635, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).
Exposure time: 60 seconds.
-
![Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)](https://www.abcam.com/ps/products/269/ab269858/Images/ab269858-4-benefits-of-recombinant-antibodies.png)
Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (ab269858)