Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19605] to Salivary alpha amylase
  • Suitable for: IHC-P, WB
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-Salivary alpha amylase antibody [EPR19605]
    See all Salivary alpha amylase primary antibodies

  • Description

    Rabbit monoclonal [EPR19605] to Salivary alpha amylase

  • Host species

    Rabbit

  • Specificity

    This product reacts with Salivary alpha amylase (P04745), Pancreatic alpha amylase (P04746) and Carcinoid alpha amylase (P19961) due to high sequence homology.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human salivary alpha-amylase fragment recombinant protein; human pancreatic alpha-amylase fragment recombinant protein; human alpha-carcinoid alpha-amylase fragment recombinant protein; human parotid gland and pancreas lysates. IHC-P: Human parotid gland and pancreas tissues

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)
    Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)
    All lanes : Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/10000 dilution

    Lane 1 : Human salivary alpha-amylase fragment recombinant protein
    Lane 2 : Human pancreatic alpha-amylase fragment recombinant protein
    Lane 3 : Human alpha-carcinoid alpha-amylase fragment recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 58 kDa
    Observed band size: 22,22,50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human salivary alpha-amylase contains aa311-511 with a GST-tag.  Human pancreatic alpha-amylase and alpha-carcinoid alpha-amylase fragment recombinant proteins contain aa311-511 with a His-Tag®.  These three recombinant proteins were made in-house.

  • Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)
    Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)
    Lanes 1-3 : Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/20000 dilution
    Lanes 4-6 : Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/1000 dilution

    Lane 1 : Human parotid gland lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : Human fetal heart lysate
    Lane 5 : Human fetal kidney lysate
    Lane 6 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 58 kDa
    Observed band size: 57 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile  observed is consistent with what has been described in the literature (PMID: 3261213).

  • Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)
    Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)
    Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/5000 dilution + Human pancreas lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 58 kDa
    Observed band size: 57 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

    Immunohistochemical analysis of paraffin-embedded human parotid gland tissue labeling salivary alpha-amylase + pancreatic alpha-amylase + alpha-carcinoid alpha-amylase with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human parotid gland is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling salivary alpha-amylase + pancreatic alpha-amylase + alpha-carcinoid alpha-amylase with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human pancreas is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

    Immunohistochemical analysis of paraffin-embedded human colon tissue with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: No staining on human colon.

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)
    Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

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