Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

100 µl 257 980 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19605] to Salivary alpha amylase
Suitable for: IHC-P, WB
Reacts with: Human, Recombinant fragment

Product name

Anti-Salivary alpha amylase antibody [EPR19605]
See all Salivary alpha amylase primary antibodies
Description

Rabbit monoclonal [EPR19605] to Salivary alpha amylase
Host species

Rabbit
Specificity

This product reacts with Salivary alpha amylase (P04745), Pancreatic alpha amylase (P04746) and Carcinoid alpha amylase (P19961) due to high sequence homology.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

Recombinant fragment

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human salivary alpha-amylase fragment recombinant protein; human pancreatic alpha-amylase fragment recombinant protein; human alpha-carcinoid alpha-amylase fragment recombinant protein; human parotid gland and pancreas lysates. IHC-P: Human parotid gland and pancreas tissues
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19605
Isotype

IgG
Research areas

Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

All lanes : Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/10000 dilution

Lane 1 : Human salivary alpha-amylase fragment recombinant protein
Lane 2 : Human pancreatic alpha-amylase fragment recombinant protein
Lane 3 : Human alpha-carcinoid alpha-amylase fragment recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 58 kDa
Observed band size: 22,22,50 kDa
why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Human salivary alpha-amylase contains aa311-511 with a GST-tag. Human pancreatic alpha-amylase and alpha-carcinoid alpha-amylase fragment recombinant proteins contain aa311-511 with a His-Tag^®. These three recombinant proteins were made in-house.
Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

Lanes 1-3 : Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/20000 dilution
Lanes 4-6 : Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/1000 dilution

Lane 1 : Human parotid gland lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Human fetal heart lysate
Lane 5 : Human fetal kidney lysate
Lane 6 : Human fetal spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 58 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID: 3261213).
Western blot - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/5000 dilution + Human pancreas lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 58 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

Immunohistochemical analysis of paraffin-embedded human parotid gland tissue labeling salivary alpha-amylase + pancreatic alpha-amylase + alpha-carcinoid alpha-amylase with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human parotid gland is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling salivary alpha-amylase + pancreatic alpha-amylase + alpha-carcinoid alpha-amylase with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human pancreas is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

Immunohistochemical analysis of paraffin-embedded human colon tissue with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control: No staining on human colon.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

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