Antibodies, Proteins, Kits and Reagents for Life Science

526 012 ₸ Product size

100 µg 526 012 ₸ 1 mg 4 690 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CD58 antibody [EPR24012-147] - BSA and Azide free (ab275398)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR24012-147] to CD58 - BSA and Azide free
Suitable for: IP, Flow Cyt, WB
Reacts with: Human

Product name

Anti-CD58 antibody [EPR24012-147] - BSA and Azide free
See all CD58 primary antibodies
Description

Rabbit monoclonal [EPR24012-147] to CD58 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IP, Flow Cyt, WBmore details
Unsuitable for: ICC or IHC-P
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Raji, K-562, Jurkat and TT whole cell lysates; Human kidney and placenta tissue lysates. Flow cyt: Jurkat and PBMC cells. IP: Raji whole cell lysate.
General notes
ab275398 is the carrier-free version of ab275392. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab275398 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C.
Storage buffer

Constituent: 100% PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24012-147
Isotype

IgG
Research areas

Flow Cytometry - Anti-CD58 antibody [EPR24012-147] - BSA and Azide free (ab275398)

This data was developed using ab275392, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD58 with ab275392 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.
Western blot - Anti-CD58 antibody [EPR24012-147] - BSA and Azide free (ab275398)

All lanes : Anti-CD58 antibody [EPR24012-147] (ab275392) at 1/1000 dilution

Lane 1 : Human kidney tissue lysate
Lane 2 : Human placenta tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

Predicted band size: 28 kDa
Observed band size: 55-70 kDa
why is the actual band size different from the predicted?

This data was developed using ab275392, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID:26039213, 3309127, 19268704).

Exposure time: 3 minutes.
Immunoprecipitation - Anti-CD58 antibody [EPR24012-147] - BSA and Azide free (ab275398)

This data was developed using ab275392, the same antibody clone in a different buffer formulation.

CD58 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab275392 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275392 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 ug

Lane 2: 275392 IP in Raji whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275392 in Raji whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 15 seconds.

This blot was developed using a higher sensitivity ECL substrate.
Western blot - Anti-CD58 antibody [EPR24012-147] - BSA and Azide free (ab275398)

All lanes : Anti-CD58 antibody [EPR24012-147] (ab275392) at 1/1000 dilution

Lane 1 : Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 40 µg
Lane 2 : A-204 (human muscle rhabdomyosarcoma) whole cell lysate at 40 µg
Lane 3 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 40 µg
Lane 4 : Jurkat (human t cell leukemia t lymphocyte) whole cell lysate at 20 µg
Lane 5 : TT (human thyroid carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

Predicted band size: 28 kDa
Observed band size: 55-70 kDa why is the actual band size different from the predicted?

This data was developed using ab275392, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID:26039213, 3309127, 19268704).

Low expression: A-204 (PMID: 26039213).

Exposure times: Lane 1: 37 seconds; Lanes 2-3: 15 seconds; Lanes 4-5: 70 seconds.
Flow Cytometry - Anti-CD58 antibody [EPR24012-147] - BSA and Azide free (ab275398)

This data was developed using ab275392, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of A-204 (Human muscle rhabdomyosarcoma, Left) / Jurkat (Human T cell leukemia T lymphocyte, Right) cells labelling CD58 with ab275392 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Low expression control: A-204.

Gated on viable cells.
Anti-CD58 antibody [EPR24012-147] - BSA and Azide free (ab275398)

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