Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IGF1 Receptor antibody [EPR23027-80] - BSA and Azide free (ab267344)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23027-80] to IGF1 Receptor - BSA and Azide free
  • Suitable for: IP, Flow Cyt, WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-IGF1 Receptor antibody [EPR23027-80] - BSA and Azide free
    See all IGF1 Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR23027-80] to IGF1 Receptor - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, Flow Cyt, WB, ICC/IFmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431, HepG2, HeLa and MDA-MB-231 lysates. ICC/IF: MCF7 cells. Flow Cyt: MCF7 cells. IP: MCF7 cells.

  • General notes

    ab267344 is the carrier-free version of ab263907. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-IGF1 Receptor antibody [EPR23027-80] - BSA and Azide free (ab267344)
    Western blot - Anti-IGF1 Receptor antibody [EPR23027-80] - BSA and Azide free (ab267344)
    All lanes : Anti-IGF1 Receptor antibody [EPR23027-80] (ab263907) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : IGF1R knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 154 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab263907).

    Lanes 1- 2: Merged signal (red and green). Green - ab263907 observed at 100 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab263907 was shown to react with IGF1 Receptor in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264801 (knockout cell lysate ab256951) was used. Wild-type HeLa and IGF1R knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab263907 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-IGF1 Receptor antibody [EPR23027-80] - BSA and Azide free (ab267344)
    Immunoprecipitation - Anti-IGF1 Receptor antibody [EPR23027-80] - BSA and Azide free (ab267344)

    IGF1 Receptor was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab263907 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263907 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10ug

    Lane 2: ab263907 IP in MCF7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263907 in MCF7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 min.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263907).

  • Flow Cytometry - Anti-IGF1 Receptor antibody [EPR23027-80] - BSA and Azide free (ab267344)
    Flow Cytometry - Anti-IGF1 Receptor antibody [EPR23027-80] - BSA and Azide free (ab267344)

    Flow cytometric analysis MCF7 (human breast adenocarcinoma epithelial cell) cells labelling IGF1 Receptor with ab263907 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263907).

  • Immunocytochemistry/ Immunofluorescence - Anti-IGF1 Receptor antibody [EPR23027-80] - BSA and Azide free (ab267344)
    Immunocytochemistry/ Immunofluorescence - Anti-IGF1 Receptor antibody [EPR23027-80] - BSA and Azide free (ab267344)

    Immunofluorescent analysis of 100% Methanol-fixed MCF7 (human breast adenocarcinoma epithelial cell) cells labelling IGF1 Receptor with ab263907 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in MCF7 cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263907).

  • Anti-IGF1 Receptor antibody [EPR23027-80] - BSA and Azide free (ab267344)
    Anti-IGF1 Receptor antibody [EPR23027-80] - BSA and Azide free (ab267344)

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