Rat IGF1 ELISA Kit (ab231924)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 15.43 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cit plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat IGF1 ELISA Kit
See all IGF1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Rat serum 8 1.7% Inter-assay Sample n Mean SD CV% Rat serum 3 2.2% -
Sample type
Serum, Tissue Extracts, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
15.43 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 80 73% - 91% Tissue Extracts 88 80% - 97% Hep Plasma 100 95% - 111% Cit plasma 87 81% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat IGF1 ELISA Kit (ab231924) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGF1 protein in hep plasma, serum, tissue extracts, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat IGF1 with 15.43 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat IGF1 Capture Antibody 1 x 600µl 10X Rat IGF1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Plate Seals 1 unit Rat IGF1 Lyophilized Recombinant Protein (ab198570) 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
Involvement in disease
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
Sequence similarities
Belongs to the insulin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IBP1
- IGF I
- IGF IA
see all -
Database links
- Entrez Gene: 24482 Rat
- SwissProt: P08025 Rat
- Unigene: 6282 Rat
Images
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Other - Rat IGF1 ELISA Kit (ab231924)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat IGF1 standard curve in Sample Diluent NS.The IGF1 standard curve was prepared as described in Section 10.
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Interpolated concentrations of native IGF1 in rat serum and plasma samples.The concentrations of IGF1 were measured in duplicates, interpolated from the IGF1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (citrate) 1:100, and plasma (heparin) 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGF1 concentration was determined to be 295.70 ng/mL in serum, 149.61 ng/mL in plasma (citrate) and 367.12 ng/mL in plasma (heparin).
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Interpolated concentrations of native IGF1 in rat heart extract based on a 1,000 µg/mL extract load.The concentrations of IGF1 were measured in duplicate and interpolated from the IGF1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGF1 concentration was determined to be 652.52 pg/mL.
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Sandwich ELISA - Rat IGF1 ELISA Kit (ab231924)To learn more about the advantages of recombinant antibodies see here.
