Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23240-64] to htrA1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free
    See all htrA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23240-64] to htrA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SK-OV-3 whole cell lysate; Human brain, placenta, ovary and ovary cancer tissue lysates. IP: Human placenta tissue lysate.

  • General notes

    ab274327 is the carrier-free version of ab274322. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23240-64

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327)
    Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327)
    All lanes : Anti-htrA1 antibody [EPR23240-64] (ab274322) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human placenta tissue lysate
    Lane 3 : Human ovary tissue lysate
    Lane 4 : Human ovary cancer tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 16767218, 19103920).

    Exposure time: 48 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274322).

  • Immunoprecipitation - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327)
    Immunoprecipitation - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327)

    htrA1 was immunoprecipitated from 0.35 mg Human placenta lysate with ab274322 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab274322 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human placenta lysate 10 ug

    Lane 2: ab274322 IP in Human placenta lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab274322 in Human placenta lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 7.75 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274322).

  • Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327)
    Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327)
    All lanes : Anti-htrA1 antibody [EPR23240-64] (ab274322) at 1/1000 dilution

    Lane 1 : SK-OV-3 (human ovarian cancer epithelial cell), whole cell lysate
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: HepG2, MCF7 (PMID: 14716297, 22761798).

    Exposure time: 3 minutes. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274322).

  • Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327)
    Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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