Anti-APPL antibody [EPR13569] - BSA and Azide free (ab250150)
![Anti-APPL antibody [EPR13569] - BSA and Azide free (ab250150)](https://www.abcam.com/ps/products/250/ab250150/Images/ab250150-409602-anti-appl-antibody-epr13569-western-blot-hela-mcf7-hek-293-lysates.jpg "Anti-APPL antibody [EPR13569] - BSA and Azide free (ab250150)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13569] to APPL - BSA and Azide free
- Suitable for: IHC-P, ICC, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-APPL antibody [EPR13569] - BSA and Azide free
See all APPL primary antibodies -
Description
Rabbit monoclonal [EPR13569] to APPL - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, MCF7 and HEK-293 cell lysates. IHC-P: Human pancreas and colon carcinoma tissue. ICC: MCF7 cells.
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General notes
Ab250150 is the carrier-free version of ab180140. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250150 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR13569 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-APPL antibody [EPR13569] - BSA and Azide free (ab250150)All lanes : Anti-APPL antibody [EPR13569] (ab180140) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : APPL knockout HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180140).
Lanes 1- 4: Merged signal (red and green). Green - ab180140 observed at 90 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab180140 was shown to react with Anti-APPL in wild-type HeLa cells in western blot. The band observed in knockout cell line ab265187 (knockout cell lysate ab257836) lane below 90kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and APPL1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab180140 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] - BSA and Azide free (ab250150)](https://www.abcam.com/ps/products/250/ab250150/Images/ab250150-2-anti-appl-antibody-epr13569-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] - BSA and Azide free (ab250150)This data was developed using ab180140, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma tissue labeling APPL with ab180140 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Immunocytochemistry - Anti-APPL antibody [EPR13569] - BSA and Azide free (ab250150)](https://www.abcam.com/ps/products/250/ab250150/Images/ab250150-3-anti-appl-antibody-epr13569-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-APPL antibody [EPR13569] - BSA and Azide free (ab250150) -
![Western blot - Anti-APPL antibody [EPR13569] - BSA and Azide free (ab250150)](https://www.abcam.com/ps/products/250/ab250150/Images/ab250150-408333-anti-appl-antibody-epr13569-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-APPL antibody [EPR13569] - BSA and Azide free (ab250150)All lanes : Anti-APPL antibody [EPR13569] (ab180140) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 80 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?This data was developed using ab180140, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] - BSA and Azide free (ab250150)](https://www.abcam.com/ps/products/250/ab250150/Images/ab250150-1-anti-appl-antibody-epr13569-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APPL antibody [EPR13569] - BSA and Azide free (ab250150)This data was developed using ab180140, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling APPL with ab180140 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Anti-APPL antibody [EPR13569] - BSA and Azide free (ab250150)](https://www.abcam.com/ps/products/250/ab250150/Images/ab250150-6-benefits-of-recombinant-antibodies.png)
Anti-APPL antibody [EPR13569] - BSA and Azide free (ab250150)