Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241] - BSA and Azide free (ab251503)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20241] to E3 ubiquitin-protein ligase MUL1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241] - BSA and Azide free
    See all E3 ubiquitin-protein ligase MUL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20241] to E3 ubiquitin-protein ligase MUL1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251503 is the carrier-free version of ab209263. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251503 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241] - BSA and Azide free (ab251503)
    Western blot - Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241] - BSA and Azide free (ab251503)
    All lanes : Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241] (ab209263) at 1/1000 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
    Lane 4 : Human fetal heart tissue lysate
    Lane 5 : Human thymus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa


    Exposure time: 15 minutes


    This data was developed using ab209263, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Negative control: Human thymus (PMID: 18591963)

  • Western blot - Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241] - BSA and Azide free (ab251503)
    Western blot - Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241] - BSA and Azide free (ab251503)
    Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241] (ab209263) at 1/1000 dilution + A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa


    Exposure time: 15 seconds


    This data was developed using ab209263, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241] - BSA and Azide free (ab251503)
    Immunoprecipitation - Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241] - BSA and Azide free (ab251503)

    This data was developed using ab209263, the same antibody clone in a different buffer formulation.

    E3 ubiquitin-protein ligase MUL1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab209263 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab209263 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate, 10 µg (Input).

    Lane 2: ab209263 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209263 in HeLa whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241] - BSA and Azide free (ab251503)
    Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241] - BSA and Azide free (ab251503)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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