Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bcl3 antibody [EPR23835-115] - BSA and Azide free (ab278529)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23835-115] to Bcl3 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Bcl3 antibody [EPR23835-115] - BSA and Azide free
    See all Bcl3 primary antibodies

  • Description

    Rabbit monoclonal [EPR23835-115] to Bcl3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, C2C12 and HeLa whole cell lysate. Mouse ovary tissue lysate.

  • General notes

    ab278529 is the carrier-free version of ab259832. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278529 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Bcl3 antibody [EPR23835-115] - BSA and Azide free (ab278529)
    Western blot - Anti-Bcl3 antibody [EPR23835-115] - BSA and Azide free (ab278529)
    All lanes : Anti-Bcl3 antibody [EPR23835-115] (ab259832) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma epithelial cell) whole cell lysate
    Lane 2 : Mouse ovary tissue lysate
    Lane 3 : C2C12 (mouse myoblasts myoblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L) Peroxidase conjugated)

    Predicted band size: 48 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab259832, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Fresh lysate was used in lane 1.

    Exposure time: Lane 1: 5.5 seconds; Lane 2-3: 3 minutes.

  • Western blot - Anti-Bcl3 antibody [EPR23835-115] - BSA and Azide free (ab278529)
    Western blot - Anti-Bcl3 antibody [EPR23835-115] - BSA and Azide free (ab278529)
    All lanes : Anti-Bcl3 antibody [EPR23835-115] (ab259832) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
    Lane 2 : THP-1 (human monocytic leukemia monocyte) whole cell lysate at 40 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 48 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    This data was developed using ab259832, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: THP-1 (PMID: 25089799).

    Exposure time: 81 seconds.

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