Anti-KMT2D / MLL2 antibody [EPR20604-73] - BSA and Azide free (ab274431)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20604-73] to KMT2D / MLL2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
-
Product name
Anti-KMT2D / MLL2 antibody [EPR20604-73] - BSA and Azide free
See all KMT2D / MLL2 primary antibodies -
Description
Rabbit monoclonal [EPR20604-73] to KMT2D / MLL2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HAP1 and HEK-293A whole cell lysates.
-
General notes
ab274431 is the carrier-free version of ab213721. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab274431 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20604-73 -
Isotype
IgG -
Research areas
Images
-
Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721) at 1/1000 dilution + HEK-293A (human embryonic kidney epithelial cell, subclone), whole cell lysate at 60 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 593 kDa
Observed band size: 600 kDa why is the actual band size different from the predicted?This data was developed using ab213721, the same antibody clone in a different buffer formulation.
The protein is loaded in Tris-Acetate gel, run at 150 V for 3 hours, then transferred on PVDF membrane at 40 V overnight. After blocking in 10% milk/TBST for 1 hour, the membrane is incubated with primary antibody overnight at 4°C.
Exposure time: 15 seconds
-
Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721) at 1/1000 dilution + HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate at 60 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 593 kDa
Observed band size: 600 kDa why is the actual band size different from the predicted?This data was developed using ab213721, the same antibody clone in a different buffer formulation.
The protein is loaded in Tris-Acetate gel, run at 150 V for 3 hours, then transferred on PVDF membrane at 40 V overnight. After blocking in 10% milk/TBST for 1 hour, the membrane is incubated with primary antibody overnight at 4°C.
Exposure time: 15 seconds
-