Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KMT2D / MLL2 antibody [EPR20604-73] - BSA and Azide free (ab274431)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20604-73] to KMT2D / MLL2 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-KMT2D / MLL2 antibody [EPR20604-73] - BSA and Azide free
    See all KMT2D / MLL2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20604-73] to KMT2D / MLL2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1 and HEK-293A whole cell lysates.

  • General notes

    ab274431 is the carrier-free version of ab213721. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab274431 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] - BSA and Azide free (ab274431)
    Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] - BSA and Azide free (ab274431)
    Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721) at 1/1000 dilution + HEK-293A (human embryonic kidney epithelial cell, subclone), whole cell lysate at 60 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 593 kDa
    Observed band size: 600 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab213721, the same antibody clone in a different buffer formulation.

    The protein is loaded in Tris-Acetate gel, run at 150 V for 3 hours, then transferred on PVDF membrane at 40 V overnight. After blocking in 10% milk/TBST for 1 hour, the membrane is incubated with primary antibody overnight at 4°C.

    Exposure time: 15 seconds

  • Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] - BSA and Azide free (ab274431)
    Western blot - Anti-KMT2D / MLL2 antibody [EPR20604-73] - BSA and Azide free (ab274431)
    Anti-KMT2D / MLL2 antibody [EPR20604-73] (ab213721) at 1/1000 dilution + HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate at 60 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 593 kDa
    Observed band size: 600 kDa why is the actual band size different from the predicted?



    This data was developed using ab213721, the same antibody clone in a different buffer formulation.

    The protein is loaded in Tris-Acetate gel, run at 150 V for 3 hours, then transferred on PVDF membrane at 40 V overnight. After blocking in 10% milk/TBST for 1 hour, the membrane is incubated with primary antibody overnight at 4°C.

    Exposure time: 15 seconds

  • Anti-KMT2D / MLL2 antibody [EPR20604-73] - BSA and Azide free (ab274431)
    Anti-KMT2D / MLL2 antibody [EPR20604-73] - BSA and Azide free (ab274431)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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