Anti-IGF1 Receptor antibody [EPR23027-204] (ab263903)
![Anti-IGF1 Receptor antibody [EPR23027-204] (ab263903)](https://www.abcam.com/ps/products/263/ab263903/Images/ab263903-2-anti-igf1-receptor-antibody-epr23027-204-immunohistochemistry-prostatic-hyperplasia-human.jpg "Anti-IGF1 Receptor antibody [EPR23027-204] (ab263903)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23027-204] to IGF1 Receptor
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-IGF1 Receptor antibody [EPR23027-204]
See all IGF1 Receptor primary antibodies -
Description
Rabbit monoclonal [EPR23027-204] to IGF1 Receptor -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431, HepG2, HeLa and MDA-MB-231 lysates. IHC-P: Human breast carcinoma, Human prostatic hyperplasia and Human bladder carcinoma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23027-204 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 Receptor antibody [EPR23027-204] (ab263903)
Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling IGF1 Receptor with ab263903 at 1/500 dilution (1.24 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Membranous and cytoplasmic staining on human prostatic hyperplasia (PMID: 20710042). The section was incubated with ab263903 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Western blot - Anti-IGF1 Receptor antibody [EPR23027-204] (ab263903)](https://www.abcam.com/ps/products/263/ab263903/Images/ab263903-4-anti-igf1-receptor-antibody-epr23027-204-western-blot-human.jpg)
Western blot - Anti-IGF1 Receptor antibody [EPR23027-204] (ab263903)All lanes : Anti-IGF1 Receptor antibody [EPR23027-204] (ab263903) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit polyclonal to GNAT2 (ab97501) at 1/100000 dilution
Predicted band size: 154 kDa
Observed band size: 130,200 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
The expression profile & molecular weight observed is consistent with what has been described in the literature (PMID: 21807868, 28591735).
Note: the bands larger than 200kDa are Pro-IGF1R
Low expression cell line: MDA-MB-231 (PMID: 28591735).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 Receptor antibody [EPR23027-204] (ab263903)](https://www.abcam.com/ps/products/263/ab263903/Images/ab263903-3-anti-igf1-receptor-antibody-epr23027-204-immunohistochemistry-bladder-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 Receptor antibody [EPR23027-204] (ab263903)Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue labeling IGF1 Receptor with ab263903 at 1/500 dilution (1.24 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Membranous and cytoplasmic staining on human bladder carcinoma (PMID: 20710042). The section was incubated with ab263903 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 Receptor antibody [EPR23027-204] (ab263903)](https://www.abcam.com/ps/products/263/ab263903/Images/ab263903-1-anti-igf1-receptor-antibody-epr23027-204-immunohistochemistry-breast-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 Receptor antibody [EPR23027-204] (ab263903)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling IGF1 Receptor with ab263903 at 1/500 dilution (1.24 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Membranous and cytoplasmic staining on human breast carcinoma (PMID: 21057462). The section was incubated with ab263903 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Anti-IGF1 Receptor antibody [EPR23027-204] (ab263903)](https://www.abcam.com/ps/products/263/ab263903/Images/ab263903-5-benefits-of-recombinant-antibodies.png)
Anti-IGF1 Receptor antibody [EPR23027-204] (ab263903)
