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Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20418] to CDKN2A/p16INK4a - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free
    See all CDKN2A/p16INK4a primary antibodies

  • Description

    Rabbit monoclonal [EPR20418] to CDKN2A/p16INK4a - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab232402 is the carrier-free version of ab211542. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232402 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)
    Flow Cytometry - Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)

    Flow cytometric analysis of 4% paraformaldehyde-fixed MEF (mouse embryonic fibroblast cell line) cell line labeling CDKN2A/p16INK4a with ab211542 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    MEF cells were kindly provided by professor Pinlong Xu, Zhejiang University.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211542).

  • Immunoprecipitation - Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)
    Immunoprecipitation - Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)

    CDKN2A/p16INK4a was immunoprecipitated from 0.35 mg of MEF (mouse embryonic fibroblast cell line) whole cell lysate with ab211542 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211542 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: MEF whole cell lysate 10 μg (Input). 

    Lane 2: ab211542 IP in MEF whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211542 in MEF whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    MEF cells were kindly provided by professor Pinlong Xu, Zhejiang University.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211542).

  • Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)
    Immunocytochemistry/ Immunofluorescence - Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)

    Immunofluorescent analysis of 100% methanol-fixed MEF (mouse embryonic fibroblast cell line) and NIH/3T3 (mouse embyro fibroblast cell line) cells labeling CDKN2A/p16INK4a with ab211542  at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on MEF cell line.

    Negative control: NIH/3T3 (PMID: 15210712).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution.

    MEF cells were kindly provided by professor Pinlong Xu, Zhejiang University.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211542).

  • Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)
    Anti-CDKN2A/p16INK4a antibody [EPR20418] - BSA and Azide free (ab232402)

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