Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CDKN2A/p16INK4a antibody - C-terminal (ab223637)

Key features and details

Goat polyclonal to CDKN2A/p16INK4a - C-terminal
Suitable for: IHC-P, WB
Reacts with: Human
Isotype: IgG

Product name

Anti-CDKN2A/p16INK4a antibody - C-terminal
See all CDKN2A/p16INK4a primary antibodies
Description

Goat polyclonal to CDKN2A/p16INK4a - C-terminal
Host species

Goat
Specificity

ab223637 is expected to recognise isoform p16INK4a (NP_000068.1) and isoform p16gamma (NP_001182061.1).
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human CDKN2A/p16INK4a aa 100 to the C-terminus (C terminal) (Cysteine residue). (NP_000068.1).
Database link: P42771

Run BLAST with Run BLAST with
Positive control
- WB: HeLa cell lysate. IHC-P: Human liver tissue.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

ab223637 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality

Polyclonal
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody - C-terminal (ab223637)

Paraffin-embedded human liver tissue stained for CDKN2A/p16INK4a using ab223637 at 5 µg/ml in immunohistochemical analysis, followed by AP staining.
Western blot - Anti-CDKN2A/p16INK4a antibody - C-terminal (ab223637)

Anti-CDKN2A/p16INK4a antibody - C-terminal (ab223637) at 1 µg/ml + HeLa cell lysate (in RIPA buffer) at 35 µg

Developed using the ECL technique.

Predicted band size: 17 kDa
Observed band size: 16-18 kDa
why is the actual band size different from the predicted?

Primary incubation was 1 hour.

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