Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ERAB antibody [EPR11839(B)] (ab167410)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11839(B)] to ERAB
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-ERAB antibody [EPR11839(B)]
    See all ERAB primary antibodies

  • Description

    Rabbit monoclonal [EPR11839(B)] to ERAB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues in Human ERAB (UniProt Q99714).

  • Positive control

    • Jurkat, 293T, K562 and HeLa whole cell lysate (ab150035); Human brain and kidney tissues; HeLa cells; K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ERAB antibody [EPR11839(B)] (ab167410)
    Western blot - Anti-ERAB antibody [EPR11839(B)] (ab167410)
    All lanes : Anti-ERAB antibody [EPR11839(B)] (ab167410) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 27 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ERAB antibody [EPR11839(B)] (ab167410)
    Immunocytochemistry/ Immunofluorescence - Anti-ERAB antibody [EPR11839(B)] (ab167410)

    Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling ERAB with purified ab167410 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAB antibody [EPR11839(B)] (ab167410)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAB antibody [EPR11839(B)] (ab167410)

    ab167410 showing +ve staining in Human heart.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAB antibody [EPR11839(B)] (ab167410)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAB antibody [EPR11839(B)] (ab167410)

    ab167410 showing +ve staining in Human gastric adenocarcinoma.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAB antibody [EPR11839(B)] (ab167410)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAB antibody [EPR11839(B)] (ab167410)

    ab167410 showing +ve staining in Human endometrial carcinoma.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAB antibody [EPR11839(B)] (ab167410)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAB antibody [EPR11839(B)] (ab167410)

    ab167410 showing +ve staining in Human normal liver.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAB antibody [EPR11839(B)] (ab167410)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAB antibody [EPR11839(B)] (ab167410)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ERAB with ab167410 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAB antibody [EPR11839(B)] (ab167410)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERAB antibody [EPR11839(B)] (ab167410)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ERAB with ab167410 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ERAB antibody [EPR11839(B)] (ab167410)
    Immunocytochemistry/ Immunofluorescence - Anti-ERAB antibody [EPR11839(B)] (ab167410)
    Immunofluorescent analysis of HeLa cells labeling ERAB with ab167410 at 1/100 dilution. DAPI nuclear staining (blue).
  • Flow Cytometry - Anti-ERAB antibody [EPR11839(B)] (ab167410)
    Flow Cytometry - Anti-ERAB antibody [EPR11839(B)] (ab167410)
    Flow cytometric analysis of permeabilized K562 cells labeling ERAB with ab167410 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
  • Anti-ERAB antibody [EPR11839(B)] (ab167410)
    Anti-ERAB antibody [EPR11839(B)] (ab167410)

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