Human Interferon beta ELISA Kit (ab278127)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2 pg/ml
- Range: 9.38 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Interferon beta ELISA Kit
See all Interferon beta kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 7% Inter-assay Sample n Mean SD CV% Serum 3 2% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2 pg/ml -
Range
9.38 pg/ml - 600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 96 83% - 106% Serum 92 89% - 97% Hep Plasma 116 112% - 119% EDTA Plasma 101 97% - 106% Cit plasma 117 114% - 120% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Interferon beta ELISA kit (ab278127) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Interferon beta protein in human serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Interferon beta with 2 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Human Interferon beta is a 166 amino acids long secreted cytokine that is produced by fibroblasts, dendritic cells, macrophages, and endothelial cells upon pathogen exposure. Interferon beta is type I interferon which mainly helps to stimulate anti-viral response. Interferon beta signals through the heterodimeric IFN-alpha/beta Receptor and activates the JAK/STAT signaling pathway.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Interferon beta Capture Antibody 1 x 600µl 10X Human Interferon beta Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Interferon beta Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Has antiviral, antibacterial and anticancer activities. -
Sequence similarities
Belongs to the alpha/beta interferon family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- beta-interferon
- Fibroblast interferon
- IFB
see all -
Database links
- Entrez Gene: 3456 Human
- Omim: 147640 Human
- SwissProt: P01574 Human
- Unigene: 93177 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Recombinant Antibody benefitsTo learn more about the advantages of recombinant antibodies see here.
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Example of human Interferon beta standard curve in Sample Diluent NS.The Interferon beta standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Interferon beta in human A549 cell culture supernatant samples transfected with 400 ng/mL or 200 ng/mL Poly I:C for 24 hours.The concentrations of Interferon beta were measured in duplicates, interpolated from the Interferon beta standard curves and corrected for sample dilution. Undiluted samples are as follows: A549 cell culture supernatant transfected with 400 ng/mL Poly I:C 100%, A549 cell culture supernatant transfected with 200 ng/mL Poly I:C 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon beta concentration was determined to be 111.4 pg/mL in neat A549 supernatant transfected with 400 ng/mL Poly I:C and 33.7 pg/mL in neat A549 supernatant transfected with 200 ng/mL Poly I:C. There was no detectable signal in mock treated A549 supernatant samples.