Mouse PON3 ELISA Kit (ab211653)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5.36 pg/ml
- Range: 0.23 ng/ml - 15 ng/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse PON3 ELISA Kit
See all PON3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 5 4% Inter-assay Sample n Mean SD CV% Plasma 3 4.1% -
Sample type
Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
5.36 pg/ml -
Range
0.23 ng/ml - 15 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 97 93% - 101% Serum 110 108% - 111% Cell culture extracts 102 101% - 104% Tissue Extracts 112 108% - 115% EDTA Plasma 109 107% - 111% Cit plasma 110 107% - 112% Fetal Bovine Serum 99 98% - 99% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse PON3 ELISA Kit (ab211653) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PON3 protein in cell culture extracts, cell culture supernatant, plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PON3 with 5.36 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Serum paraoxonase/lactonase 3 (PON3) is a calcium binding 354 amino acid protein. PON3 possesses hydrolytic catalytic activity against aromatic and 5- or 6- member ring lactones. PON3 is a member of the paraoxonase family consisting of PON1 and PON, which are involved in modulating oxidative stress and possess anti-atherogenic properties. PON3 is a secreted protein that associates with high-density lipoprotein (HDL).
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse PON3 Capture Antibody 1 x 600µl 10X Mouse PON3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse PON3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Has low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents. -
Sequence similarities
Belongs to the paraoxonase family. -
Post-translational
modificationsThe signal sequence is not cleaved. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Alternative names
- 2810004E20
- AI786302
- MGC95026
see all -
Database links
- Entrez Gene: 269823 Mouse
- SwissProt: Q62087 Mouse
- Unigene: 9122 Mouse
Images
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Other - Mouse PON3 ELISA Kit (ab211653)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse PON3 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked recombinant PON3 in pooled samples.Interpolated concentrations of spiked recombinant PON3 in pooled normal mouse serum, plasma (citrate), and cell culture media (RPMI 1640 + 10% fetal bovine serum) samples, and native PON3 in plasma (EDTA) samples. The concentrations of PON3 were measured in duplicate, interpolated from the PON3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (EDTA) 20% and cell culture media 32.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native PON3 in RAW 264.7 cell extract and spiked recombinant PON3 in mouse liver extract based on a 1,000 µg/mL extract load.The concentrations of PON3 were measured in duplicate and interpolated from the PON3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Native levels of mouse PON3 as measured by SimpleStep ELISA (mean +/- SD).Dilutions of pooled normal mouse serum and plasma within the linear range were measured in duplicate, their background-subtracted values interpolated off of the mouse PON3 standard curve, and corrected for dilution back to neat. The mean PON3 concentration was determined to be 6.7 ng/mL in serum, 4.5 ng/mL in plasma (citrate), 17.8 ng/mL in plasma (EDTA), and 6 ng/mL in RAW 264.7 cell culture supernatant.
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Native levels of mouse PON3 as measured by SimpleStep ELISA (mean +/- SD).Dilutions of cell and tissue extracts within the linear range were measured in duplicate, their background-subtracted values interpolated off of the mouse PON3 standard curve, and corrected for dilution back to a 1000 µg extract load. The mean PON3 concentration was determined to be 71.9 ng/mg in liver extract and 669 ng/mg in RAW 264.7 cell extract.
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Sandwich ELISA - Mouse PON3 ELISA Kit (ab211653)To learn more about the advantages of recombinant antibodies see here.
