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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Mouse PON3 Matched Antibody Pair Kit (ab217615)

Mouse PON3 Matched Antibody Pair Kit (ab217615)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Mouse
  • Range: 187.5 pg/ml - 12000 pg/ml

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Overview

  • Product name

    Mouse PON3 Matched Antibody Pair Kit
    See all PON3 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Sensitivity

    56.1 pg/ml
  • Range

    187.5 pg/ml - 12000 pg/ml
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Mouse PON3 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse PON3.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab211653. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 5 x 96 tests
    Mouse PON3 Capture Antibody 2 x 50µg 1 x 50µg
    Mouse PON3 Detector Antibody 2 x 12.5µg 1 x 12.5µg
    Mouse PON3 Lyophilized Protein 2 vials 1 vial
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Other
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
  • Function

    Has low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents.
  • Sequence similarities

    Belongs to the paraoxonase family.
  • Post-translational
    modifications

    The signal sequence is not cleaved.
  • Cellular localization

    Secreted > extracellular space.
  • Target information above from: UniProt accession Q15166 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 2810004E20
    • AI786302
    • MGC95026
    • Paraoxonase 3
    • Pon3
    • PON3_HUMAN
    • Serum paraoxonase/lactonase 3
    see all
  • Database links

    • Entrez Gene: 269823 Mouse
    • SwissProt: Q62087 Mouse
    • Unigene: 9122 Mouse

    Images

    • Mouse PON3 standard curve.
      Mouse PON3 standard curve.

      Standard calibration curve. Background subtracted values are graphed.

    • Sandwich ELISA - Mouse PON3 Matched Antibody Pair Kit (ab217615)
      Sandwich ELISA - Mouse PON3 Matched Antibody Pair Kit (ab217615)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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