Mouse PON3 Matched Antibody Pair Kit (ab217615)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 187.5 pg/ml - 12000 pg/ml
Overview
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Product name
Mouse PON3 Matched Antibody Pair Kit
See all PON3 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
56.1 pg/ml -
Range
187.5 pg/ml - 12000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse PON3 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse PON3.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab211653. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse PON3 Capture Antibody 2 x 50µg 1 x 50µg Mouse PON3 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse PON3 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Has low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents. -
Sequence similarities
Belongs to the paraoxonase family. -
Post-translational
modificationsThe signal sequence is not cleaved. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Alternative names
- 2810004E20
- AI786302
- MGC95026
see all -
Database links
- Entrez Gene: 269823 Mouse
- SwissProt: Q62087 Mouse
- Unigene: 9122 Mouse
Images
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Standard calibration curve. Background subtracted values are graphed.
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To learn more about the advantages of recombinant antibodies see here.