Anti-NCKAP1 antibody (ab226303)
Key features and details
- Rabbit polyclonal to NCKAP1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NCKAP1 antibody
See all NCKAP1 primary antibodies -
Description
Rabbit polyclonal to NCKAP1 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Orangutan -
Immunogen
Synthetic peptide within Human NCKAP1 aa 925-975. The exact sequence is proprietary.
Database link: Q9Y2A7 -
Positive control
- IP: HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab226303 was affinity purified using an epitope specific to NCKAP1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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NCKAP1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer: 1 mg for IP, 20% of IP loaded) with ab226303 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226303 at 0.4 µg/ml.
Lane 1: ab226303 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.