Anti-Endothelium antibody [EN4] (ab8087)
Key features and details
- Mouse monoclonal [EN4] to Endothelium
- Reacts with: Guinea pig, Cat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Endothelium antibody [EN4]
See all Endothelium primary antibodies -
Description
Mouse monoclonal [EN4] to Endothelium -
Host species
Mouse -
Specificity
This antibody stains all human blood vessels including brain microvessels. Both large and small vessels are equally reactive. The EN4 antigen is preserved in endothelial cells in culture unlike the PAL-E antigen (ab8086) which is lost. It stains strongly murine fibroblasts transfected with the human CD31 gene. On surface-iodinated Jurkat T cells it recognizes the 130 kD CD31 antigen. EN4 also binds to guinea-pig and cat endothelium, but not to rabbit, bovine, sheep, dog, rat or mice endothelial cells. No other structures in the skin, heart, kidney, tonsils or spleen are stained with this antibody. -
Species reactivity
Reacts with: Guinea pig, Cat, Human
Does not react with: Mouse, Sheep, Rabbit, Cow, Dog -
General notes
The antibody is useful for immunohistological staining of endothelia. Lymphatic endothelium is stained (unlike the PAL-E antibody). Kaposi's sarcoma and other vascular tumors of endothelial cell origin are also stained.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Primary antibody notes
The antibody is useful for immunohistological staining of endothelia. Lymphatic endothelium is stained (unlike the PAL-E antibody). Kaposi's sarcoma and other vascular tumors of endothelial cell origin are also stained. -
Clonality
Monoclonal -
Clone number
EN4 -
Isotype
IgG1 -
Research areas
