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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Cancer Tumor biomarkers Enzymes Phosphatases

Anti-GADD34 antibody (ab175355)

Anti-GADD34 antibody (ab175355)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to GADD34
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-GADD34 antibody
    See all GADD34 primary antibodies
  • Description

    Rabbit polyclonal to GADD34
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human GADD34 aa 595-612.
    Sequence:

    RITQAQEELSPCLTPAAR


    Database link: O75807
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa cell lysate; HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Phosphatases
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Other
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Phospatases

Images

  • Western blot - Anti-GADD34 antibody (ab175355)
    Western blot - Anti-GADD34 antibody (ab175355)
    Lane 2 : Anti-GADD34 antibody (ab175355) at 1/250 dilution
    Lanes 3 & 5 : Anti-GADD34 antibody (ab175355) at 1/500 dilution
    Lane 4 : Anti-GADD34 antibody (ab175355) at 1/1000 dilution

    Lanes 2-4 : HeLa cell lysate
    Lane 5 : HeLa cell lysate with neutralizing peptide

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG-HRP secondary antibody

    Developed using the ECL technique.

    Predicted band size: 73 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-GADD34 antibody (ab175355)
    Immunocytochemistry/ Immunofluorescence - Anti-GADD34 antibody (ab175355)

    Immunofluorescent analysis of formalin-fixed HeLa cells labeling GADD34 with ab175355 at 1/50 and incubated with DyLight 488 Goat anti-rabbit IgG secondary antibody. F-actin (red) was stained with DyLight 650 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-GADD34 antibody (ab175355)

  •  
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    Anti-GADD34 antibody (ab9869)

    Applications: IHC-P, WB

  •  
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    Anti-GADD34 antibody [OTI2B11] (ab236516)

    Applications: Flow Cyt, ICC/IF, WB

  •  
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    Anti-GADD34 antibody (ab126075)

    Applications: WB

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