Anti-ERAB antibody (ab137455)
Key features and details
- Rabbit polyclonal to ERAB
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ERAB antibody
See all ERAB primary antibodies -
Description
Rabbit polyclonal to ERAB -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to a region within amino acids 201-211 of Human ERAB.
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Positive control
- HeLaS3, MOLT4, Neuro 2A, GL261 and PC12 whole cell lysates; A549 cells; OVCA xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ERAB antibody (ab137455) at 1/1000 dilution
Lane 1 : HeLa S3 whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa
15% SDS PAGE -
All lanes : Anti-ERAB antibody (ab137455) at 1/1000 dilution
Lane 1 : Neuro 2A whole cell lysate
Lane 2 : GL261 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa
12% SDS PAGE -
Anti-ERAB antibody (ab137455) at 1/5000 dilution + PC12 whole cell lysate at 30 µg
Predicted band size: 27 kDa
12% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde fixed A549 cells, labelling ERAB using ab137455 at 1/200 dilution. Right panel costained with Hoerchst 33342.
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Immunohistochemical analysis of paraffin embedded OVCA xenograft tissue, labelling ERAB using ab137455 at 1/100 dilution.