Anti-ERAB antibody (ab237806)
Key features and details
- Rabbit polyclonal to ERAB
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ERAB antibody
See all ERAB primary antibodies -
Description
Rabbit polyclonal to ERAB -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant full length protein corresponding to Human ERAB aa 1 to the C-terminus.
Database link: Q99714 -
Positive control
- WB: HEK-293T, HepG2, HeLa, Jurkat and HT-29 whole cell lysates; Mouse brain lysate. IP: HEK-293T whole cell lysate. ICC/IF: A549 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity greater than 95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ERAB antibody (ab237806) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : HeLa (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 27 kDa
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A549 (human lung carcinoma cell line) cells stained for ERAB (green) using ab237806 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counter stain is DAPI.
The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.
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All lanes : Anti-ERAB antibody (ab237806) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 27 kDa
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ERAB was immunoprecipitated from 0.5 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with ab237806 at 1/200 dilution.
Lane 1: Control rabbit IgG IP in HEK-293T whole cell lysate.
Lane 2: ab237806 IP in HEK-293T whole cell lysate.
Lane 3: HEK-293T whole cell lysate 10 μg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.