Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-RAB39B antibody [EPR9651] (ab154826)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9651] to RAB39B
Suitable for: ICC, WB, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-RAB39B antibody [EPR9651]
See all RAB39B primary antibodies
Description

Rabbit monoclonal [EPR9651] to RAB39B
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Mouse Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human RAB39B. The exact sequence is proprietary.
Database link: Q96DA2
Positive control
- WB: Human fetal brain tissue lysate and U87-MG and SH-SY5Y cell lysates; ICC: SH-SY5Y and mouse primary neuron cells; Flow Cyt: U87-MG cells.
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9651
Isotype

IgG
Research areas

Western blot - Anti-RAB39B antibody [EPR9651] (ab154826)

All lanes : Anti-RAB39B antibody [EPR9651] (ab154826) at 1/100 dilution (unpurified)

Lane 1 : Human fetal brain tissue lysate
Lane 2 : U87-MG cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Immunocytochemistry - Anti-RAB39B antibody [EPR9651] (ab154826)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling RAB39B with ab154826 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining in mouse primary neuron cell.Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).
Flow Cytometry - Anti-RAB39B antibody [EPR9651] (ab154826)

Flow cytometry analysis of U87-MG cells labelling RAB39B with unpurified ab154826 (red) at 1/9. Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.
Western blot - Anti-RAB39B antibody [EPR9651] (ab154826)

All lanes : Anti-RAB39B antibody [EPR9651] (ab154826) at 1/2000 dilution (purified)

Lane 1 : Human fetal brain tissue lysate
Lane 2 : U87-MG cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-RAB39B antibody [EPR9651] (ab154826)

All lanes : Anti-RAB39B antibody [EPR9651] (ab154826) at 1/1000 dilution (unpurified)

Lane 1 : Fetal brain lysate
Lane 2 : U87-MG cell lysate
Lane 3 : SH-SY5Y cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 25 kDa
Immunocytochemistry - Anti-RAB39B antibody [EPR9651] (ab154826)

Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling RAB39B with unpurified ab154826 at 1/10 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
Immunocytochemistry - Anti-RAB39B antibody [EPR9651] (ab154826)

Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling RAB39B with purified ab154826 at 1/200 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).
Flow Cytometry - Anti-RAB39B antibody [EPR9651] (ab154826)

Flow cytometry analysis of U87-MG cells labelling RAB39B with purified ab154826 (red) at 1/210. Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.
Anti-RAB39B antibody [EPR9651] (ab154826)

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