Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR069G] to BRD3
Suitable for: IP, WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-BRD3 antibody [BLR069G]
See all BRD3 primary antibodies
Description

Rabbit monoclonal [BLR069G] to BRD3
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human BRD3 aa 676-726. The exact sequence is proprietary. NP_031397.1.
Database link: Q15059
Positive control
- IHC-P: Human lung carcinoma and colon carcinoma tissues. ICC: OVCAR-3 cells. WB: HEK-293T, RKO, Jurkat, LNCaP, HeLa, MCF7, K562 and HepG2 whole cell lysates. IP: HEK-293T whole cell lysate.
General notes

This product is sold under License from Bethyl Laboratories, Inc

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Borate buffered saline
Concentration information loading...
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR069G
Isotype

IgG
Research areas
- Epigenetics and Nuclear Signaling
- Bromodomains

Western blot - Anti-BRD3 antibody [BLR069G] (ab264420)

All lanes : Anti-BRD3 antibody [BLR069G] (ab264420) at 1/1000 dilution

Lane 1 : HEK-293T whole cell lysate
Lane 2 : RKO whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : LNcaP whole cell lysate
Lane 5 : HeLa whole cell lysate
Lane 6 : MCF7 whole cell lysate
Lane 7 : K562 whole cell lysate
Lane 8 : HepG2 whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Developed using the ECL technique.

Predicted band size: 80 kDa

Exposure time: 10 seconds

Lysates prepared using NETN lysis buffer.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (ab264420)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling BRD3 with ab264420 at 1/200 dilution. Detection: DAB. Secondary: DyLight^® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).
Immunocytochemistry/ Immunofluorescence - Anti-BRD3 antibody [BLR069G] (ab264420)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 cells labeling BRD3 with ab264420 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (ab264420)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling BRD3 with ab264420 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunoprecipitation - Anti-BRD3 antibody [BLR069G] (ab264420)

BRD3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264420 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitates using ab264420 at 1/1000 dilution.

Lane 1: ab264420 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.

Detection: Chemiluminescence with an exposure time of 1 second.

Lysates prepared using NETN lysis buffer.
Anti-BRD3 antibody [BLR069G] (ab264420)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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