Anti-BRD3 antibody [BLR069G] (ab264420)
![Anti-BRD3 antibody [BLR069G] (ab264420)](https://www.abcam.com/ps/products/264/ab264420/Images/ab264420-366135-AntiBRD3-antibody-BLR069G-western-blot-hek293t-rko-jurkat-hela-mcf7-k562-hepg2-human.jpg "Anti-BRD3 antibody [BLR069G] (ab264420)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR069G] to BRD3
- Suitable for: IP, WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-BRD3 antibody [BLR069G]
See all BRD3 primary antibodies -
Description
Rabbit monoclonal [BLR069G] to BRD3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanIP HumanWB Human
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Immunogen
Synthetic peptide within Human BRD3 aa 676-726. The exact sequence is proprietary. NP_031397.1.
Database link: Q15059 -
Positive control
- IHC-P: Human lung carcinoma and colon carcinoma tissues. ICC: OVCAR-3 cells. WB: HEK-293T, RKO, Jurkat, LNCaP, HeLa, MCF7, K562 and HepG2 whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
This product is sold under License from Bethyl Laboratories, Inc
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Borate buffered saline -
Concentration information loading... -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR069G -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BRD3 antibody [BLR069G] (ab264420)All lanes : Anti-BRD3 antibody [BLR069G] (ab264420) at 1/1000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : RKO whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : LNcaP whole cell lysate
Lane 5 : HeLa whole cell lysate
Lane 6 : MCF7 whole cell lysate
Lane 7 : K562 whole cell lysate
Lane 8 : HepG2 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 80 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (ab264420)](https://www.abcam.com/ps/products/264/ab264420/Images/ab264420-2-anti-brd3-antibody-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (ab264420)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling BRD3 with ab264420 at 1/200 dilution. Detection: DAB. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-BRD3 antibody [BLR069G] (ab264420)](https://www.abcam.com/ps/products/264/ab264420/Images/ab264420-3-anti-brd3-antibody-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BRD3 antibody [BLR069G] (ab264420)Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 cells labeling BRD3 with ab264420 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (ab264420)](https://www.abcam.com/ps/products/264/ab264420/Images/ab264420-1-anti-brd3-antibody-immunohistochemistry-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] (ab264420)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling BRD3 with ab264420 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
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![Immunoprecipitation - Anti-BRD3 antibody [BLR069G] (ab264420)](https://www.abcam.com/ps/products/264/ab264420/Images/ab264420-366134-AntiBRD3-antibody-BLR069G-immunoprecipitation-hek293t-human.jpg)
Immunoprecipitation - Anti-BRD3 antibody [BLR069G] (ab264420)BRD3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264420 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitates using ab264420 at 1/1000 dilution.
Lane 1: ab264420 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with an exposure time of 1 second.
Lysates prepared using NETN lysis buffer.
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![Anti-BRD3 antibody [BLR069G] (ab264420)](https://www.abcam.com/ps/products/264/ab264420/Images/ab264420-6-benefits-of-recombinant-antibodies.png)
Anti-BRD3 antibody [BLR069G] (ab264420)
