Anti-BRD3 antibody [2088C3a] (ab50818)
![Anti-BRD3 antibody [2088C3a] (ab50818)](https://www.abcam.com/ps/products/50/ab50818/Images/ab50818-397675-anti-brd3-antibody-2088c3a-western-blot-human-knockout-lysate.jpg "Anti-BRD3 antibody [2088C3a] (ab50818)")
Key features and details
- Mouse monoclonal [2088C3a] to BRD3
- Suitable for: Flow Cyt, WB, IP, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-BRD3 antibody [2088C3a]
See all BRD3 primary antibodies -
Description
Mouse monoclonal [2088C3a] to BRD3 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIP HumanWB MouseRatHuman
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Immunogen
Recombinant fragment of BRD3 (Human)
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Positive control
- WB: HEK293T, Jurkat, HeLa, F2408 and NIH3T3 whole cell lysates. ICC/IF and Flow Cyt: HeLa cells. IP: RIPA lysate of HeLa cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
2088C3a -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)All lanes : Anti-BRD3 antibody [2088C3a] (ab50818) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : BRD3 knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 80 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab50818 observed at 100 kDa. Red - loading control ab181602 observed at 36 kDa.
ab50818 Anti-BRD3 antibody [2088C3a] was shown to specifically react with BRD3 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266793 (knockout cell lysate ab258335) was used. Wild-type and BRD3 knockout samples were subjected to SDS-PAGE. ab50818 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-BRD3 antibody [2088C3a] (ab50818)](https://www.abcam.com/ps/products/50/ab50818/Images/ab50818-62507-anti-brd3-antibody-2088c3a-immunoprecipitation.jpg)
Immunoprecipitation - Anti-BRD3 antibody [2088C3a] (ab50818)Immunoprecipitation of BRD3 from RIPA lysate of HeLa cells (500µg) using 5µg ab50818. Visualized by western blot using 1/50 ab50518 as primary and anti-mouse IgG secondary (1:2500). Predicted band size 80 KDa. -
![Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)](https://www.abcam.com/ps/products/50/ab50818/Images/ab50818-62511-anti-brd3-antibody-2088c3a-western-blot.jpg)
Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)Anti-BRD3 antibody [2088C3a] (ab50818) at 1/200 dilution + F2408 whole cell lysate at 50 µg
Secondary
Anti Mouse IgG antibody at 1/2500 dilution
Predicted band size: 80 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)](https://www.abcam.com/ps/products/50/ab50818/Images/ab50818-62510-anti-brd3-antibody-2088c3a-western-blot.jpg)
Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)Anti-BRD3 antibody [2088C3a] (ab50818) at 1/50 dilution + HeLa whole cell lysate at 50 µg
Secondary
Anti-Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 80 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)](https://www.abcam.com/ps/products/50/ab50818/Images/ab50818-62509-anti-brd3-antibody-2088c3a-western-blot.jpg)
Western blot - Anti-BRD3 antibody [2088C3a] (ab50818)Anti-BRD3 antibody [2088C3a] (ab50818) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg
Secondary
Anti-Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 80 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
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![Immunocytochemistry/ Immunofluorescence - Anti-BRD3 antibody [2088C3a] (ab50818)](https://www.abcam.com/ps/products/50/ab50818/Images/ab50818-62508-anti-brd3-antibody-2088c3a-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BRD3 antibody [2088C3a] (ab50818)Immunoflourescent staining of Hela cells using ab50818 Anti-BRD3 Antibody (1/10 dilution) as primary and AlexaFluor®488 Goat Anti-mouse IgG( 1/200 dilution ) -
![Flow Cytometry - Anti-BRD3 antibody [2088C3a] (ab50818)](https://www.abcam.com/ps/products/50/ab50818/Images/ab50818-62506-anti-brd3-antibody-2088c3a-flow-cytometry.jpg)
Flow Cytometry - Anti-BRD3 antibody [2088C3a] (ab50818)Flow Cytometric analysis using ab50818 (0.5ug). Secondary antibody used: AlexaFluor®488 Goat Anti-mouse IgG (0.25 µg)
