Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR069G] to BRD3 - BSA free
  • Suitable for: WB, IP, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-BRD3 antibody [BLR069G] - BSA free
    See all BRD3 primary antibodies

  • Description

    Rabbit monoclonal [BLR069G] to BRD3 - BSA free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human BRD3 aa 676-726. The exact sequence is proprietary. NP_031397.1.
    Database link: Q15059

  • Positive control

    • IHC-P: Human lung carcinoma and colon carcinoma tissues. ICC: OVCAR-3 cells. IP: HEK-293T whole cell lysate.

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

    ab272081 is the carrier-free version of ab264420. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Properties

Images

  • Immunoprecipitation - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)
    Immunoprecipitation - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)

    BRD3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264420 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitates using ab264420 at 1/1000 dilution.

    Lane 1: ab264420 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 1 second.

    Lysates prepared using NETN lysis buffer.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264420).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling BRD3 with ab264420 at 1/200 dilution. Detection: DAB. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264420).

  • Immunocytochemistry/ Immunofluorescence - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)
    Immunocytochemistry/ Immunofluorescence - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)

    Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 cells labeling BRD3 with ab264420 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264420).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling BRD3 with ab264420 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264420).

  • Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)
    Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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