Antibodies, Proteins, Kits and Reagents for Life Science

Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR069G] to BRD3 - BSA free
Suitable for: WB, IP, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-BRD3 antibody [BLR069G] - BSA free
See all BRD3 primary antibodies
Description

Rabbit monoclonal [BLR069G] to BRD3 - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human

See all applications and species data

Immunogen

Synthetic peptide within Human BRD3 aa 676-726. The exact sequence is proprietary. NP_031397.1.
Database link: Q15059
Positive control
- IHC-P: Human lung carcinoma and colon carcinoma tissues. ICC: OVCAR-3 cells. IP: HEK-293T whole cell lysate.
General notes

This product is sold under License from Bethyl Laboratories, Inc.

ab272081 is the carrier-free version of ab264420. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline
Concentration information loading...
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR069G
Isotype

IgG
Research areas
- Epigenetics and Nuclear Signaling
- Bromodomains

Immunoprecipitation - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)

BRD3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264420 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitates using ab264420 at 1/1000 dilution.
Lane 1: ab264420 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 1 second.
Lysates prepared using NETN lysis buffer.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264420).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling BRD3 with ab264420 at 1/200 dilution. Detection: DAB. Secondary: DyLight^® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264420).
Immunocytochemistry/ Immunofluorescence - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 cells labeling BRD3 with ab264420 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264420).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling BRD3 with ab264420 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab264420).
Anti-BRD3 antibody [BLR069G] - BSA free (ab272081)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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