Anti-KSR1 antibody [EPR2421Y] (ab68483)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2421Y] to KSR1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-KSR1 antibody [EPR2421Y]
See all KSR1 primary antibodies -
Description
Rabbit monoclonal [EPR2421Y] to KSR1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseIHC-P HumanWB MouseRatHuman -
Immunogen
A synthetic peptide corresponding to residues near the C-terminus of human KSR.
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Positive control
- WB: Wild-type HAP1 cell lysate. HeLa, MEF, Rat1 and HEK-293 cell lysate. Flow Cyt: NIH/3T3 cells. IHC-P: Human cervical carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2421Y -
Isotype
IgG -
Research areas
Images
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Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: KSR1 knockout HAP1 cell lysate (40 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab68483 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.Unpurified ab68483 was shown to recognize KSR1 when KSR1 knockout samples were used, along with additional cross-reactive bands. Wild-type and KSR1 knockout samples were subjected to SDS-PAGE. Ab68483 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue sections labeling KSR1 with Purified ab68483 at 1:100 dilution (1.49 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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Unpurified ab68483 staining KSR1 in NIH/3T3 (mouse embryo) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/230. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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Anti-KSR1 antibody [EPR2421Y] (ab68483) at 1/1000 dilution (purified) + MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
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Anti-KSR1 antibody [EPR2421Y] (ab68483) at 1/1000 dilution (purified) + HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
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Anti-KSR1 antibody [EPR2421Y] (ab68483) at 1/1000 dilution (purified) + Rat1 (Rat embryonic fibroblast) whole cell lysates
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 102 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
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Anti-KSR1 antibody [EPR2421Y] (ab68483) at 1/2000 dilution (unpurified) + HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 10 µg
Secondary
goat anti rabbit HRP at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
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