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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111)

Price and availability

251 280 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2359(2)] to KSR1 (phospho S392)
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-KSR1 (phospho S392) antibody [EPR2359(2)]
    See all KSR1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2359(2)] to KSR1 (phospho S392)
  • Host species

    Rabbit
  • Specificity

    ab129111 only detects KSR1 phosphorylated at Serine 392.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human KSR1 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • Calyculin A-treated 293T cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 8.00 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2359(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Images

  • Western blot - Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111)
    Western blot - Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111)
    All lanes : Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111) at 1/1000 dilution (purified)

    Lane 1 : Untreated HEK293 cells
    Lane 2 : HEK293 cells treated with Calcyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 102 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111)
    Western blot - Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111)
    All lanes : Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111) at 1/1000 dilution (unpurified)

    Lane 1 : 293T cell lysate, untreated
    Lane 2 : 293T cell lysate treated with Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 102 kDa

  • OI-RD Scanning - Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111)
    OI-RD Scanning - Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111)
    Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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