Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2359(2)] to KSR1 (phospho S392)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-KSR1 (phospho S392) antibody [EPR2359(2)]
See all KSR1 primary antibodies -
Description
Rabbit monoclonal [EPR2359(2)] to KSR1 (phospho S392) -
Host species
Rabbit -
Specificity
ab129111 only detects KSR1 phosphorylated at Serine 392. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide within Human KSR1 (internal sequence). The exact sequence is proprietary.
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Positive control
- Calyculin A-treated 293T cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 8.00 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2359(2) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111) at 1/1000 dilution (purified)
Lane 1 : Untreated HEK293 cells
Lane 2 : HEK293 cells treated with Calcyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-KSR1 (phospho S392) antibody [EPR2359(2)] (ab129111) at 1/1000 dilution (unpurified)
Lane 1 : 293T cell lysate, untreated
Lane 2 : 293T cell lysate treated with Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
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